FB 2, Mikrobiologie, Universität Bremen, Leobener Str., D-2800, Bremen 33, FRG.
Microb Ecol. 1988 Jul;16(1):17-29. doi: 10.1007/BF02097402.
In this paper we present a method of isolation and morphological and physiological characterization of groundwater bacteria based on numerical taxonomy and cluster analysis, and using a miniaturized test system (microtiter plates). Bacteria were isolated randomly on P-agar, and each strain was characterized in regard to 155 features. The media for biochemical differentiation are listed as well as methods of morphological discrimination. 246 strains of heterotrophic and oligotrophic bacteria, isolated from five water samples from different depths of the saturated groundwater area, were used for optimizing media and test reactions.
本文介绍了一种基于数值分类和聚类分析的地下水细菌的分离方法和形态学及生理学特性研究，并采用微型测试系统（微量滴定板）。在 P 琼脂上随机分离细菌，对每个菌株的 155 个特征进行了描述。本文列出了生化分化的培养基以及形态鉴别方法。从饱和地下水区域不同深度的 5 个水样中分离出 246 株异养菌和寡养菌，用于优化培养基和测试反应。
