Yoshikawa Kozo, Shimada Mitsuo, Kuwahara Tomomi, Hirakawa Hideki, Kurita Nobuhiro, Sato Hirohiko, Utsunomiya Tohru, Iwata Takashi, Miyatani Tomohiko, Higashijima Jun, Kashihara Hideya, Takasu Chie, Matsumoto Noriko, Nakayama-Imaohji Haruyuki
Department of Surgery, Institute of Health Biosciences, the University of Tokushima Graduate School.
J Med Invest. 2013;60(3-4):221-7. doi: 10.2152/jmi.60.221.
Diversity of gut microbiome has been recently reported to be lost in inflammatory bowel disease. We have previously reported that the Dai-kenchu-to (DKT) prevented the bacterial translocation through suppression of cytokine and apoptosis in rat's fast stress model. The aim of this study was to evaluate the effect of DKT on maintenance of microbial diversity in rat's intestine with inflammation.
Wister rats were received the fast stress for 5 days. In DKT group, rats were administered with DKT (300 mg/kg/day) during the fast stress (DKT-group). The gut microbiomes were analyzed at before- and after- fast stress, and the effect of DKT for on microbial diversities of the gut were evaluated by the PCR-clone library method targeting the 16 S ribosomal RNA gene.
In Control-group, Erysipelotrichaceae increased to 86% in after fast stress, OTU of before-fast stress was 111 and after fast stress was only 9 (changing rate: 58%). The diversity of microbiome was severely decreased. On the other hand, in DKT-group, diversity of microbiome was kept after fast stress (Lachnospiraceae: Ruminococcaceae: Coriobacteriales 54%, 22%, 5%), Operational taxonomic units of before fast stress was 52 and after fast stress was 55 (changing rate: 6%). Family Lachnospiraceae which includes butyrate-producing Clostridia (Clostridium IV and XIVa).
DKT prevented the reduction of diversity of microbiome in rat's fast stress model. Our data suggested the new anti-inflammatory mechanism of DKT through gut microbiome.
最近有报道称炎症性肠病患者肠道微生物群的多样性丧失。我们之前曾报道，大建中汤（DKT）在大鼠快速应激模型中通过抑制细胞因子和细胞凋亡来预防细菌易位。本研究的目的是评估DKT对炎症状态下大鼠肠道微生物多样性维持的影响。
将Wistar大鼠进行5天的快速应激。在DKT组中，大鼠在快速应激期间给予DKT（300mg/kg/天）（DKT组）。在快速应激前后分析肠道微生物群，并通过针对16S核糖体RNA基因的PCR克隆文库法评估DKT对肠道微生物多样性的影响。
在对照组中，快速应激后丹毒丝菌科增加到86%，快速应激前的操作分类单元（OTU）为111，快速应激后仅为9（变化率：58%）。微生物群的多样性严重降低。另一方面，在DKT组中，快速应激后微生物群的多样性得以保持（毛螺菌科：瘤胃球菌科：棒状杆菌目54%、22%、5%），快速应激前的操作分类单元为52，快速应激后为55（变化率：6%）。毛螺菌科包括产生丁酸盐的梭菌（梭菌属IV和XIVa）。
DKT在大鼠快速应激模型中可防止微生物群多样性的降低。我们的数据提示了DKT通过肠道微生物群发挥的新抗炎机制。
