Yoshikawa Kozo, Kurita Nobuhiro, Higashijima Jun, Miyatani Tomohiko, Miyamoto Hidenori, Nishioka Masanori, Shimada Mitsuo
Department of Surgery, Institute of Health Biosciences, The University of Tokushima Graduate School, 3-18-15 Kuramoto, Tokushima-city, Tokushima 770-8503, Japan.
Dig Dis Sci. 2008 Jul;53(7):1824-31. doi: 10.1007/s10620-008-0281-3. Epub 2008 Apr 30.
Kampo medicine "Dai-kenchu-to" (DKT) has been used for treatment of ileus. The aim of this study was to evaluate the role of DKT on the bacterial translocation (BT) model in rats. Rats were divided into the following four groups: group 1, receiving only water, and groups 2, 3, and 4, receiving 100, 300, and 1,000 mg/kg/day of DKT. Rats were sacrificed 6 days after the beginning of the fast, and then the mesenteric lymph node was cultured. Inflammatory cytokines, intestinal integrity, and apoptosis were assessed. Incidence of BT in groups 3 (33%) and 4 (16%) was lower than in group 1 (66%). Interferon-gamma expression in groups 2, 3, and 4 was significantly lower than in group 1. Villous height and number of villus in groups 2, 3, and 4 were significantly taller and greater than in group 1. Apoptotic index in groups 2, 3, and 4 was significantly lower than in group 1. This is the first evidence that DKT prevents BT by reducing inflammatory reaction and maintaining intestinal integrity.
汉方药物“大建中汤”（DKT）已被用于治疗肠梗阻。本研究的目的是评估DKT在大鼠细菌移位（BT）模型中的作用。将大鼠分为以下四组：第1组，仅给予水；第2、3、4组，分别给予100、300和1000mg/kg/天的DKT。禁食开始6天后处死大鼠，然后培养肠系膜淋巴结。评估炎性细胞因子、肠道完整性和细胞凋亡情况。第3组（33%）和第4组（16%）的BT发生率低于第1组（66%）。第2、3、4组的干扰素-γ表达明显低于第1组。第2、3、4组的绒毛高度和绒毛数量明显高于且多于第1组。第2、3、4组的凋亡指数明显低于第1组。这是DKT通过减轻炎症反应和维持肠道完整性来预防BT的首个证据。
