Department of Crop Protection, Waite Campus, The University of Adelaide, PMB, 1, 5064, Glen Osmond, S.A., Australia.
Theor Appl Genet. 1993 Dec;87(5):598-602. doi: 10.1007/BF00221884.
The mode of inheritance of resistance to bipyridyl herbicides in bipyridyl-resistant biotypes of Arctotheca calendula and of Hordeum leporinum was investigated. F1 plants from reciprocal crosses between diquat-resistant and -susceptible plants of A. calendula showed an intermediate response to diquat application that was nuclearly inherited. Treatment of F2 plants with 100 g ai ha(-1) of diquat or 800 g ai ha(-1) of paraquat killed all homozygous-susceptible plants, caused severe injury to heterozygous plants but only slight or no injury to homozygous-resistant plants. Back crosses of F1 to susceptible plants exhibited intermediate and susceptible phenotypes. The observed segregation ratios in F2 and test-cross populations fitted predicted segregation ratios, 1:2:1 (R:I:S) and 1:1 (I:S) respectively, showing that bipyridyl resistance is conferred by a single incompletely-dominant gene. Biotypes of paraquat-resistant and -susceptible H. leporinum were crossed reciprocally. F1 plants from reciprocal crosses showed an intermediate response to paraquat application. The F2 progeny showed segregation ratios that fitted the predicted segregation ratio of 1:2:1 (R:I:S) forinheritance of resistance being governed by a single partially-dominant gene.
我们研究了月见草和黑麦中对双吡啶类除草剂具有抗性的生物型的抗性遗传方式。从月见草对二氯喹啉酸具有抗性和敏感性的植株之间进行正反交得到的 F1 代植株对二氯喹啉酸的反应为中间型，这种反应是核遗传的。用 100 g ai ha(-1) 的二氯喹啉酸或 800 g ai ha(-1) 的百草枯处理 F2 代植株，可杀死所有纯合敏感植株，使杂合植株受到严重伤害，但对纯合抗性植株只造成轻微或无伤害。F1 与敏感植株的回交表现出中间型和敏感表型。在 F2 代和测交群体中观察到的分离比例分别符合预期的 1:2:1（R：I：S）和 1:1（I：S）分离比例，表明双吡啶类除草剂抗性由一个不完全显性单基因决定。对具有对百草枯抗性和敏感性的黑麦生物型进行了正反交。来自正反交的 F1 代植株对百草枯的应用表现出中间型反应。F2 代的分离比例符合预期的 1:2:1（R：I：S）分离比例，表明抗性由一个部分显性单基因控制。
