Dang Cécile, de Montaudouin Xavier, Binias Cindy, Salvo Flora, Caill-Milly Nathalie, Bald Juan, Soudant Philippe
The University of Queensland, School of Biological Science and Centre for Marine Science, Brisbane, Queensland 4072, Australia.
Dis Aquat Organ. 2013 Nov 6;106(3):255-65. doi: 10.3354/dao02640.
Perkinsosis is one of the most widespread diseases affecting commercially important species of molluscs globally. We examined the impact of Perkinsus spp. on shell growth at the individual scale in 2 clam species: Ruditapes decussatus from Mundaka Estuary (Spain) and R. philippinarum from Arcachon Bay (France). At Arcachon, 2 contrasting sites in terms of environment and Perkinsus olseni presence were chosen: Arguin (disease-free) and Ile aux Oiseaux (infected site). We monitored the dynamics of perkinsosis over the course of the experiment at Mundaka and Ile aux Oiseaux. Prevalences were high (>70%), and intensities were around 105 cells g-1 wet gills at Ile aux Oiseaux, and 106 cells g-1 at Mundaka. No significant differences in prevalence or intensity were observed over time. A 2 yr field growth experiment of tagged-recaptured clams was performed to determine individual clam growth rate, condition index (CI), and Perkinsus spp. infection intensity. Clams were collected at Ile aux Oiseaux and transplanted to Arguin. The growth rate was always significantly and negatively correlated with Perkinsus spp. infection, and positively correlated with CI. CI and Perkinsus spp. infection explained 19% and 7% of the variability of the growth rate at Mundaka and Ile aux Oiseaux, respectively. In experimental clams at Arguin, P. olseni infection explained 26% of the variability of the growth rate at the lower tidal level. Our results suggest that at a concentration of between 105 and 106 cells g-1, perkinsosis affects the physiological functions of the clams, highlighted by its impact on the growth rate.
派金虫病是全球影响重要商业贝类物种的最广泛传播的疾病之一。我们在个体尺度上研究了派金虫属对两种蛤类贝壳生长的影响：来自西班牙蒙达卡河口的四角蛤蜊和来自法国阿卡雄湾的菲律宾蛤仔。在阿卡雄，选择了两个在环境和奥尔森派金虫存在方面形成对比的地点：阿尔金（无病）和鸟类岛（感染地点）。我们在蒙达卡和鸟类岛的实验过程中监测了派金虫病的动态。鸟类岛的患病率很高（>70%），感染强度约为每克湿鳃105个细胞，蒙达卡为每克106个细胞。随着时间的推移，患病率或感染强度没有观察到显著差异。进行了一项为期两年的标记重捕蛤类的野外生长实验，以确定个体蛤类的生长速率、状况指数（CI）和派金虫属的感染强度。蛤类在鸟类岛采集并移植到阿尔金。生长速率始终与派金虫属感染呈显著负相关，与CI呈正相关。CI和派金虫属感染分别解释了蒙达卡和鸟类岛生长速率变异性的19%和7%。在阿尔金的实验蛤类中，奥尔森派金虫感染解释了低潮位时生长速率变异性的26%。我们的结果表明，当浓度在每克105至106个细胞之间时，派金虫病会影响蛤类的生理功能，这在其对生长速率的影响中得到了突出体现。
