Manipal Institute of Regenerative Medicine, Manipal University, Bangalore, India.
Cells Tissues Organs. 2013;198(3):198-208. doi: 10.1159/000354901. Epub 2013 Oct 26.
The therapeutic potential of mesenchymal stromal cells depends on their ability to survive and proliferate under adverse in vivo scenarios in a particular disease. In most of the sites of injury, especially in diabetic wounds, there can be hypoxia, hyperglycemia, and ischemia, leading to a lack of nutrients. Hence, the aim of our present study was to investigate the influence of hypoxia, high glucose, and low serum concentrations on the growth kinetics and proliferative potential of human dental pulp stem cells from exfoliated deciduous teeth (SHED) and permanent teeth (DPSC). In this study we isolated two types of specialized stem cells from human dental pulp tissues, which were supposedly of neural crest origin, and cultured them in KO-DMEM medium supplemented with 10% fetal bovine serum (FBS). Both SHED and DPSC were characterized for standard CD surface markers, and their ability to differentiate into adipogenic and osteogenic lineages was tested. SHED and DPSC were exposed to either hypoxia or high glucose or low serum conditions, and their growth kinetics and differentiation potentials were compared with those of normal culture conditions. We found that SHED retained their phenotypic expression and differentiation potential under hypoxia, high-glucose, and low-serum conditions and exhibited a higher proliferation in terms of cell yield and a reduced doubling time compared to DPSC. Our findings clearly demonstrate for the first time that SHED are superior to DPSC as evidenced by their enhanced proliferation under adverse culture conditions.
间充质基质细胞的治疗潜力取决于其在特定疾病的不利体内情况下存活和增殖的能力。在大多数损伤部位，特别是在糖尿病伤口中，可能存在缺氧、高血糖和缺血，导致营养物质缺乏。因此，我们目前的研究旨在研究缺氧、高血糖和低血清浓度对脱落乳牙（SHED）和恒牙（DPSC）来源的人牙髓基质干细胞生长动力学和增殖潜力的影响。在这项研究中，我们从牙髓组织中分离出两种类型的专门干细胞，这些干细胞被认为来源于神经嵴，并在补充有 10%胎牛血清（FBS）的 KO-DMEM 培养基中培养。我们对 SHED 和 DPSC 进行了标准 CD 表面标志物的鉴定，并测试了它们向成脂和成骨谱系分化的能力。SHED 和 DPSC 分别暴露于缺氧、高糖或低血清条件下，比较其生长动力学和分化潜能与正常培养条件下的情况。我们发现 SHED 在缺氧、高糖和低血清条件下保持其表型表达和分化潜能，并且与 DPSC 相比，细胞产量更高，倍增时间更短，增殖能力更强。我们的研究结果首次清楚地表明，SHED 在不利的培养条件下比 DPSC 具有更强的增殖能力。
