Al-Hadi Mona Abdulrahman Abdullah
Faculty of Dentistry, Airlangga University, Surabaya, Indonesia.
Faculty of Dentistry, University of Science and Technology, Sana'a, Yemen.
BDJ Open. 2024 Mar 13;10(1):21. doi: 10.1038/s41405-024-00197-5.
Bone diseases have a profound global impact, especially when the body's innate regenerative capacity falls short in the face of extensive damage. Stem cells from human exfoliated deciduous teeth (SHEDs), discovered in 2003, offer a promising solution for tissue repair, as they self-renew naturally and are easily obtainable. Mesenchymal stem cells (MSCs), including SHEDs, are believed to promote tissue regeneration by releasing growth factors, collectively known as the secretome.
This study explored the potential of combining SHED-derived secretome with Yemeni Sidr honey to improve osteoblast and fibroblast cell viability and migration.
The experiment involved treating cell cultures of two types of rat cell lines - 7F2 osteoblast and BHK-21 fibroblast immortalized cells - with SHED-derived secretome and Yemeni Sidr honey. After the treatment, cell viability was measured using the MTT assay, which calculates OD at 590 nm. Additionally, the scratch assay was conducted to evaluate cell migration, and ImageJ software was used for data processing.
The findings indicated that combining SHED-derived secretome and Yemeni Sidr honey enhanced osteoblast and fibroblast cell viability and migration. Furthermore, the study highlighted the difference in the stimulative potential of SHED-derived secretome, Yemeni Sidr honey, and their combination, on the viability and migration of the cultured cells.
The research concludes that combining SHED-derived secretome with Yemeni Sidr honey has the potential to promote cell viability and migration in in-vitro settings. The synergistic application of these substances has been found to be more effective -when combined in a dose-dependent manner- than their counterparts. Overall, the current study serves as a foundation for further investigations to establish if the explored substance has any useful clinical applications.
骨疾病对全球有着深远影响，尤其是当面对广泛损伤时人体的天然再生能力不足时。2003年发现的人脱落乳牙干细胞（SHEDs）为组织修复提供了一个有前景的解决方案，因为它们能自然自我更新且易于获取。包括SHEDs在内的间充质干细胞（MSCs）被认为通过释放统称为分泌组的生长因子来促进组织再生。
本研究探讨将SHEDs来源的分泌组与也门 Sidr 蜂蜜结合以提高成骨细胞和成纤维细胞活力及迁移能力的潜力。
该实验涉及用SHEDs来源的分泌组和也门Sidr蜂蜜处理两种大鼠细胞系——7F2成骨细胞和永生化的BHK - 21成纤维细胞的细胞培养物。处理后，使用MTT法测量细胞活力，该方法计算590nm处的光密度（OD）。此外，进行划痕试验以评估细胞迁移，并使用ImageJ软件进行数据处理。
研究结果表明，将SHEDs来源的分泌组与也门Sidr蜂蜜结合可提高成骨细胞和成纤维细胞的活力及迁移能力。此外，该研究还突出了SHEDs来源的分泌组、也门Sidr蜂蜜及其组合对培养细胞活力和迁移的刺激潜力的差异。
该研究得出结论，将SHEDs来源的分泌组与也门Sidr蜂蜜结合有潜力在体外环境中促进细胞活力和迁移。已发现这些物质以剂量依赖方式联合使用时的协同应用比单独使用更有效。总体而言，本研究为进一步调查所探索的物质是否有任何有用的临床应用奠定了基础。
