Kurup V P, John K V, Resnick A, Fink J N
Int Arch Allergy Appl Immunol. 1986;79(3):263-9. doi: 10.1159/000233984.
Culture filtrate antigens of Aspergillus fumigatus were fractionated by isoelectric focusing using a pH gradient of 4-6.5. Three fractions, namely, 18, 19 and 20 were pooled and subjected to immunochemical analysis. It contained a concanavalin A binding glycoprotein. Antibody raised against this component was used to prepare an affinity column of IgG-Sepharose and was used to purify crude culture filtrate antigens. This component produced three precipitin arcs in crossed immunoelectrophoresis using anti-A. fumigatus rabbit serum. This fraction has an isoelectric point of 6.5 and showed three components in two-dimensional electrophoresis with approximate molecular weights of 20, 40 and 80 kilo daltons. This antigen reacted with patient sera in the biotin-avidin linked immunosorbent assay and showed high levels of anti-A. fumigatus IgG and IgE antibodies in allergic bronchopulmonary aspergillosis and IgG antibodies in aspergilloma. Both controls and Aspergillus skin test positive asthmatics showed only low levels of specific antibodies. Because of the purity of this antigen, its potential use as a standardized antigen in the detection of antibody is discussed.
烟曲霉的培养滤液抗原通过使用pH梯度为4 - 6.5的等电聚焦进行分级分离。将三个级分，即18、19和20合并，并进行免疫化学分析。它含有一种伴刀豆球蛋白A结合糖蛋白。针对该成分产生的抗体用于制备IgG - 琼脂糖亲和柱，并用于纯化粗培养滤液抗原。该成分在使用抗烟曲霉兔血清的交叉免疫电泳中产生三条沉淀弧。该级分的等电点为6.5，在二维电泳中显示出三种成分，其近似分子量分别为20、40和80千道尔顿。该抗原在生物素 - 抗生物素蛋白连接免疫吸附试验中与患者血清发生反应，在变应性支气管肺曲霉病中显示出高水平的抗烟曲霉IgG和IgE抗体，在曲霉肿中显示出IgG抗体。对照和曲霉皮肤试验阳性的哮喘患者仅显示低水平的特异性抗体。由于该抗原的纯度，讨论了其作为标准化抗原在抗体检测中的潜在用途。
