López-Medrano R, Ovejero M C, Calera J A, Puente P, Leal F
Departamento de Microbiología y Genética, Universidad de Salamanca, Consejo Superior de Investigaciones Científica, Spain.
Infect Immun. 1995 Dec;63(12):4774-80. doi: 10.1128/iai.63.12.4774-4780.1995.
We have identified, purified, and characterized structurally and functionally a 90-kDa immunodominant antigen associated with the water-soluble fraction of Aspergillus fumigatus. This antigen is recognized by 90.3% of serum samples from patients with aspergilloma and should be considered either by itself or better in combination with other purified antigens as a candidate for developing a standardized immunoassay for the detection of aspergilloma. p90 is a glycoprotein containing at least two two N-linked sugar chains of 2 and 5 kDa, respectively, which are not necessary for its reactivity with aspergilloma serum samples. Using specific anti-p90 rabbit serum, we have demonstrated that under native conditions, p90 exists in oligomeric form and has associated catalase activity. This activity is resistant to extreme temperatures (> 60 degrees C), reducing agents (40 mM dithiothreitol), high concentrations of denaturing agents such as 8 M urea and 8% sodium dodecyl sulfate, and treatments with ethanol-chloroform-water (5:3:10 [vol/vol]) mixtures.
我们已经鉴定、纯化并在结构和功能上表征了一种与烟曲霉水溶性部分相关的90 kDa免疫显性抗原。90.3%的曲菌球患者血清样本可识别该抗原，应单独或更好地与其他纯化抗原结合考虑，将其作为开发用于检测曲菌球的标准化免疫测定的候选物。p90是一种糖蛋白，分别含有至少两条2 kDa和5 kDa的N-连接糖链，这些糖链对于其与曲菌球血清样本的反应性并非必需。使用特异性抗p90兔血清，我们已经证明在天然条件下，p90以寡聚体形式存在并具有相关的过氧化氢酶活性。该活性对极端温度（>60摄氏度）、还原剂（40 mM二硫苏糖醇）、高浓度变性剂如8 M尿素和8%十二烷基硫酸钠以及用乙醇-氯仿-水（5:3:10 [体积/体积]）混合物处理具有抗性。
