Neurath A R, Kent S B, Strick N
J Gen Virol. 1986 Mar;67 ( Pt 3):453-61. doi: 10.1099/0022-1317-67-3-453.
Amino acid sequences coded for by the preS region of the hepatitis B virus (HBV) envelope gene are present both in HBV and in subviral hepatitis B surface antigen (HBsAg) particles. Consequently, anti-preS-specific antibodies are elicited during the course of HBV infection. Such antibodies are virus-neutralizing. Therefore, it is important to determine whether or not vaccination with HBsAg also induces an anti-preS-specific immune response. We describe here an enzyme-linked immunosorbent assay applicable for the screening of sera from vaccinated individuals for anti-preS antibodies. IgG from serum specimens was adsorbed to staphylococcal Protein A on a superparamagnetic support and subsequently mixed with a synthetic peptide analogue [preS(120-145)] covalently linked to beta-lactamase. The presence of anti-preS in serum specimens resulted in binding of the conjugated beta-lactamase to the magnetic support. The adsorbed enzyme was quantified colorimetrically.
乙型肝炎病毒（HBV）包膜基因前S区编码的氨基酸序列既存在于HBV中，也存在于亚病毒乙型肝炎表面抗原（HBsAg）颗粒中。因此，在HBV感染过程中会引发抗前S特异性抗体。此类抗体具有病毒中和作用。所以，确定接种HBsAg疫苗是否也能诱导抗前S特异性免疫反应非常重要。我们在此描述一种酶联免疫吸附测定法，适用于筛查接种疫苗个体血清中的抗前S抗体。血清标本中的IgG吸附到超顺磁性载体上的葡萄球菌蛋白A上，随后与共价连接到β-内酰胺酶的合成肽类似物[preS(120 - 145)]混合。血清标本中抗前S的存在导致共轭β-内酰胺酶与磁性载体结合。通过比色法对吸附的酶进行定量。
