Department of Pharmacognosy, Royal Danish School of Pharmacy, Universitetsparken 2, DK-2100, Copenhagen, Denmark.
Plant Cell Rep. 1993 Jul;12(9):517-20. doi: 10.1007/BF00236099.
Cell cultures from different species of the genus Thapsia (Apiaceae) have been investigated. In one 4-yearold line of T. garganica L. spontaneous somatic embryogenesis up to the globular stage occurred in a suspension culture containing 1 mg l(-1)2,4-dichlorophenoxyacetic acid (2,4-D). Also callus cultures of this line, previously maintained on a medium containing 1 mg l(-1) 2,4-D, when transferred to various media deprived of 2,4-D, produced somatic embryos that developed into plantlets. Cell culture, embryos and regenerated organs were analysed for their content of thapsigargins. The undifferentiated cell culture did not synthezise thapsigargins, but was found to produce a yet unidentified compound not present in planta. White embryos in the pre-cotyledonary stage did not synthezise thapsigargins either, but when the embryos developed to the cotyledonary stage and became green, the synthesis started. Regenerated roots and shoots also contained thapsigargins.
已经对属的不同物种的细胞培养物进行了研究。在一个 4 岁的 T. garganica L. 线中，在含有 1 mg l(-1)2,4-二氯苯氧乙酸（2,4-D）的悬浮培养物中自发发生了球形胚状体。该系的愈伤组织培养物以前在含有 1 mg l(-1) 2,4-D 的培养基上维持，当转移到各种不含 2,4-D 的培养基上时，产生了体细胞胚，这些胚发育成小植株。对细胞培养物、胚胎和再生器官进行了分析，以确定它们的含量。未分化的细胞培养物不合成紫杉，而是发现产生了一种在植物中不存在的尚未鉴定的化合物。在子叶前阶段的白色胚胎也不合成紫杉，但当胚胎发育到子叶阶段并变成绿色时，合成就开始了。再生的根和茎也含有紫杉。
