Department of Genetics, University of California, Berkeley, Berkeley, California 94720.
Plant Physiol. 1986 Aug;81(4):1143-6. doi: 10.1104/pp.81.4.1143.
Embryogenesis in a wild carrot cell line, W001C, can start and progress up to the first morphogenetic stage (the globular-stage embryo) in 2,4-dichloropenoxyacetic acid (2,4-D). To clarify the quantitative effect of 2,4-D on this cell line, morphological and biochemical criteria have been used to monitor embryogenesis in the presence of increasing concentrations of 2,4-D. The biochemical criteria are the ability to inactivate cycloheximide and the expression of an embryogenic polypeptide E(1). The results show that 2,4-D can affect embryogenesis in a quantitative manner but never fully suppresses embryogenesis unless it is coupled with high cell density.
在野胡萝卜细胞系 W001C 中，胚胎发生可以在 2,4-二氯苯氧乙酸（2,4-D）中启动并进展到第一个形态发生阶段（球形胚胎）。为了阐明 2,4-D 对该细胞系的定量影响，使用形态学和生物化学标准来监测存在不同浓度 2,4-D 时的胚胎发生。生化标准是失活环己酰亚胺的能力和胚胎发生多肽 E(1)的表达。结果表明，2,4-D 可以以定量方式影响胚胎发生，但除非与高细胞密度相结合，否则永远不会完全抑制胚胎发生。
