Central Research Laboratories, Ajinomoto Co. Inc., 1-1 Suzuki-cho, 210, Kawasaki, Japan.
Theor Appl Genet. 1993 Feb;85(8):969-75. doi: 10.1007/BF00215036.
Interspecific hybrids between Allium cepa and Allium sativum were obtained using the fertile clone A. sativum as the male parent. The nascent embryos which formed shortly in interspecific hybridization between A. cepa and A. sativum were rescued by ovule culture at an early stage. The zygotes or proembryos developed in Murashige and Skoog medium containing 5.7×10(-8) M indole-3-butyric acid (IBA). Once developed, the embryos were taken out of the ovule and cultured on embryo culture medium where they regenerated into whole plants. The hybridity of the plants obtained was examined by morphological observation, chromosome analysis, and ribosomal RNA gene analysis. The analyses proved that the plants were mature sexual hybrids between A. cepa and A. sativum. Each hybrid plant had keeled but fistulose leaves and formed a bulb resembling that of A. cepa. The hybrids produced not only S-propenyl-L-cysteine sulfoxide, which is the major flavor precursor in A. cepa, but also S-allyl-L-cysteine sulfoxide (alliin), which is characteristic of A. sativum.
采用可育无性系 A. sativum 作为雄性亲本，通过洋葱与大蒜的种间杂交获得种间杂种。在洋葱和大蒜的种间杂交中，早期通过胚珠培养挽救了短时间内形成的初生胚胎。在含有 5.7×10（-8）M 吲哚-3-丁酸（IBA）的 Murashige 和 Skoog 培养基中发育的受精卵或原胚。一旦发育，将胚胎从胚珠中取出，并在胚胎培养基上培养，在那里它们再生为完整的植物。通过形态观察、染色体分析和核糖体 RNA 基因分析检查获得的植物的杂种性。分析证明，这些植物是洋葱和大蒜之间成熟的有性杂种。每个杂种植物都有龙骨状但拳头状的叶子，并形成类似于洋葱的鳞茎。杂种不仅产生 S-丙烯基-L-半胱氨酸亚砜，这是洋葱中的主要风味前体，而且还产生 S-烯丙基-L-半胱氨酸亚砜（蒜氨酸），这是大蒜特有的。
