Department of Biological Sciences, University of Exeter, Washington-Singer-Labs, Perry Road, EX4 4QG, Exeter, England.
Theor Appl Genet. 1985 Sep;70(6):655-60. doi: 10.1007/BF00252292.
Analyses of leaves and 'tubers' from somatic hybrids of potato and tomato ('pomato' with plastids of potato, 'topato' with plastids of tomato) produced by fusion of protoplasts from liquid cultures of dihaploid potato and mesophyll of tomato revealed the presence of the two major potato glycoalkaloids (α-solanine and α-chaconine) as well as the tomato glycoalkaloid (αtomatine). The total alkaloid content of leaves was greater than that of 'tubers' and similar to levels in the foliage of parent plants. However, glycoalkaloids were more abundant in hybrid 'tubers' than in normal potato tubers by a factor of 5-15. In hybrid foliage, approximately 98% of the alkaloid present was of potato origin whereas in 'tubers' the reverse was the case, with tomatine comprising 60-70% of the total alkaloid. The similarities in alkaloid content and ratios between the pomato and the topato lines indicate that plastomes do not influence the biosynthesis and distribution of these alkaloids. The results indicate that major secondary metabolites may prove useful for assessing the hybrid nature of such plants.
对由原生质体融合产生的体细胞杂种（具有马铃薯质体的“pomato”，具有番茄质体的“topato”）的叶片和“块茎”进行分析，发现存在两种主要的马铃薯糖苷生物碱（α-茄碱和α-卡茄碱）以及番茄糖苷生物碱（α-番茄碱）。叶片中的总生物碱含量大于“块茎”，与亲本植物叶片中的水平相似。然而，与正常马铃薯块茎相比，杂种“块茎”中的糖苷生物碱含量高出 5-15 倍。在杂种叶片中，约 98%的生物碱来源于马铃薯，而在“块茎”中则相反，番茄碱占总生物碱的 60-70%。pomato 和 topato 系之间生物碱含量和比例的相似性表明质体不会影响这些生物碱的生物合成和分布。研究结果表明，主要的次生代谢物可能有助于评估此类植物的杂种性质。
