Biotechnology Group, Danish Research Service for Plant and Soil Science, Lottenborgvej 2, DK-2800, Lyngby, Denmark.
Theor Appl Genet. 1993 Feb;85(8):994-1000. doi: 10.1007/BF00215039.
The Brassica napus genome has been investigated by DNA fingerprinting with six synthetic oligonucleotide probes complementary to simple repetitive sequences, namely (GATA)4, (GACA)4, (GGAT)4, (CA)8, (CT)8 and (GTG)5. While all sequence motifs were found to be present in the B. napus genome, their organization and abundance varied considerably. Among the investigated probes, (GATA)4 revealed the highest level of intraspecific polymorphism and distinguishes not only between cultivars but even between different individuals belonging to the same cultivar. In contrast, (GTG)5, (GACA)4 and (GGAT)4 produced relatively homogeneous fingerprint patterns throughout different cultivars, while hybridization to (CT)8 and (CA)8 resulted in only a few weak bands superimposed on a smear. The isoschizomeric pair Hpa II and Msp I revealed the presence of methylated cytosines in the vicinity of (GATA)m repeats. The applicability of simple repetitive sequence polymorphisms as molecular markers for Brassica species is discussed.
通过用 6 种与简单重复序列互补的合成寡核苷酸探针（GATA）4、（GACA）4、（GGAT）4、（CA）8、（CT）8 和（GTG）5 对油菜的基因组进行 DNA 指纹分析，研究了油菜的基因组。虽然所有的序列基序都存在于油菜基因组中，但它们的组织和丰度有很大的差异。在所研究的探针中，（GATA）4 显示出最高水平的种内多态性，不仅可以区分不同品种，甚至可以区分同一品种的不同个体。相比之下，（GTG）5、（GACA）4 和（GGAT）4 在不同品种中产生相对均匀的指纹模式，而与（CT）8 和（CA）8 的杂交仅导致少数弱带叠加在弥散带之上。同裂酶 Hpa II 和 Msp I 显示（GATA）m 重复附近存在甲基化胞嘧啶。讨论了简单重复序列多态性作为芸薹属物种分子标记的适用性。
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