Gupta V S, Ramakrishna W, Rawat S R, Ranjekar P K
Plant Molecular Biology Unit, National Chemical Laboratory, Pune, India.
Biochem Genet. 1994 Feb;32(1-2):1-8. doi: 10.1007/BF00557234.
In the present report the potential of (CAC)5 is demonstrated for DNA fingerprinting in rice. Based on the fingerprint data, (CAC)5 was estimated to differentiate up to 6.3 x 10(8) genotypes of rice. In a search for homologous sequences in rice transcripts, four oligonucleotide probes, namely, (CAC)5, (GATA)4, (GACA)4, and (TG)10, were used. Among these probes, (CAC)5 showed hybridization to total rice RNA in a tissue-specific manner; while a band at 1.2 kb was common in both seed and leaf RNA, there were additional prominent bands at 1.9 and 2.3 kb in seed and leaf RNA, respectively. When (CAC)5 was hybridized to poly(A)+ RNA, in addition to a band at 1.2 kb in seed and leaf, hybridization was observed only in seed to heterogeneously sized RNAs.
在本报告中，证明了（CAC）5在水稻DNA指纹分析中的潜力。基于指纹数据，估计（CAC）5可区分多达6.3×10⁸种水稻基因型。在寻找水稻转录本中的同源序列时，使用了四种寡核苷酸探针，即（CAC）5、（GATA）4、（GACA）4和（TG）10。在这些探针中，（CAC）5以组织特异性方式与水稻总RNA杂交；虽然1.2 kb处的条带在种子和叶片RNA中都很常见，但在种子和叶片RNA中分别还有1.9 kb和2.3 kb处的额外突出条带。当（CAC）5与聚腺苷酸加尾RNA杂交时，除了种子和叶片中1.2 kb处的条带外，仅在种子中观察到与大小不均一的RNA杂交。
