Plant Genetics Section, Environmental Science and Technology Department, Risø National Laboratory, DK-4000, Roskilde, Denmark.
Theor Appl Genet. 1995 Jul;91(2):236-41. doi: 10.1007/BF00220883.
We have examined the inheritance of 20 rapeseed (Brassica napus)-specific RAPD (randomly amplified polymorphic DNA) markers from transgenic, herbicide-tolerant rapeseed in 54 plants of the BC1 generation from the cross B. junceax(B. junceaxB. napus). Hybridization between B. juncea and B. napus, with B. juncea as the female parent, was successful both in controlled crosses and spontaneously in the field. The controlled backcrossing of selected hybrids to B. juncea, again with B. juncea as the female parent, also resulted in many seeds. The BC1 plants contained from 0 to 20 of the rapeseed RAPD markers, and the frequency of inheritance of individual RAPD markers ranged from 19% to 93%. The transgene was found in 52% of the plants analyzed. Five synteny groups of RAPD markers were identified. In the hybrids pollen fertility was 0-28%. The hybrids with the highest pollen fertility were selected as male parents for backcrossing, and pollen fertility in the BC1 plants was improved (24-90%) compared to that of the hybrids.
我们检查了来自转基因、耐除草剂油菜的 20 个油菜特异性 RAPD（随机扩增多态性 DNA）标记在 B. junceax(B. junceaxB. napus)杂交的 BC1 代 54 株植物中的遗传情况。B. juncea 和 B. napus 之间的杂交，以 B. juncea 为母本，无论是在控制杂交还是在田间自然杂交中都很成功。选择的杂种与 B. juncea 的再次回交，同样以 B. juncea 为母本，也产生了许多种子。BC1 植物含有 0 到 20 个油菜 RAPD 标记，单个 RAPD 标记的遗传频率范围从 19%到 93%。分析的植物中有 52%发现了转基因。鉴定了 5 个 RAPD 标记的同线群。杂种的花粉育性为 0-28%。花粉育性最高的杂种被选为回交的父本，与杂种相比，BC1 植物的花粉育性得到了提高（24-90%）。
