Shillitoe E J, Hwang C B, Silverman S, Greenspan J S
J Natl Cancer Inst. 1986 Mar;76(3):371-4.
Although patients with oral cancer have increased levels of antibody to herpes simplex virus type 1, the origin of the antigenic stimulation remains unknown. We have therefore looked for proteins of herpes simplex in oral squamous cell carcinomas by staining frozen sections with monoclonal antibodies to the proteins ICP 4, ICP 5, ICP 6, ICP 8, and gB. No staining was seen of the tumor cells of any of 11 oral cancer cases or of the epithelium of 29 other oral lesions, which included cases of leukoplakia, lichen planus, and aphthous ulcers. Frequent staining of mast cells was seen in the connective tissue associated with oral cancer when ascitic fluid was used as the source of monoclonal antibody, but such staining was not seen when the precipitated IgG fraction was used.
尽管口腔癌患者体内抗1型单纯疱疹病毒抗体水平升高，但抗原刺激的来源仍不清楚。因此，我们通过用针对ICP 4、ICP 5、ICP 6、ICP 8和gB蛋白的单克隆抗体对冰冻切片进行染色，来寻找口腔鳞状细胞癌中的单纯疱疹病毒蛋白。在11例口腔癌病例的肿瘤细胞以及29例其他口腔病变（包括白斑、扁平苔藓和复发性口腔溃疡）的上皮细胞中均未观察到染色。当使用腹水作为单克隆抗体来源时，在与口腔癌相关的结缔组织中可见肥大细胞频繁染色，但使用沉淀的IgG组分时则未观察到这种染色。
