Kassim K H, Daley T D
University of Western Ontario, Canada.
Oral Surg Oral Med Oral Pathol. 1988 Apr;65(4):445-8. doi: 10.1016/0030-4220(88)90359-3.
Although considerable evidence has accumulated linking herpes simplex virus (HSV) to carcinoma of the uterine cervix, only fragmentary evidence exists linking HSV to oral cancer. The purpose of this study was to search for HSV type 1 (HSV-1) proteins in tissue sections of 132 oral squamous cell carcinomas and 56 age- and site-matched controls. All tissues were incubated in rabbit anti-HSV-1 serum with the use of a modified direct immunoperoxidase technique. More than 42% of the cancers, but none of the controls, reacted positively. Thirteen selected oral carcinomas that exhibited positive polyclonal staining were incubated with the use of a peroxidase-antiperoxidase technique with a mouse monoclonal antibody raised against the 89 kilodalton HSV-1 envelope protein. None of these tissues exhibited a positive reaction. Similarly, no unequivocal ultrastructural viral particles were found in 11 oral carcinomas that stained positively with the polyclonal antibodies. These results are interpreted to indicate partial expression of a noninfectious viral genome. The presence of this genome in a high proportion of lesions in this study lends strong statistical support to the oncogenic relationship between HSV-1 and oral squamous cell carcinoma.
尽管已有大量证据表明单纯疱疹病毒（HSV）与子宫颈癌有关，但将HSV与口腔癌联系起来的证据却很零散。本研究的目的是在132例口腔鳞状细胞癌组织切片和56例年龄及部位匹配的对照组织切片中寻找1型单纯疱疹病毒（HSV-1）蛋白。使用改良的直接免疫过氧化物酶技术，将所有组织与兔抗HSV-1血清一起孵育。超过42%的癌症组织呈阳性反应，而对照组织均无阳性反应。对13例表现出多克隆染色阳性的口腔癌组织，使用针对89千道尔顿HSV-1包膜蛋白的小鼠单克隆抗体，采用过氧化物酶-抗过氧化物酶技术进行孵育。这些组织均未呈现阳性反应。同样，在11例经多克隆抗体染色呈阳性的口腔癌组织中，未发现明确的超微结构病毒颗粒。这些结果被解释为表明存在非感染性病毒基因组的部分表达。本研究中高比例病变组织中该基因组的存在，为HSV-1与口腔鳞状细胞癌之间的致癌关系提供了有力的统计学支持。
