Centre for Biosciences, Jamia Millia Islamia, 110 025, New Delhi, India.
Plant Cell Rep. 1994 Aug;13(11):619-22. doi: 10.1007/BF00232932.
Factors involved in promoting caulogenesis from hypocotyl explants of Phyllanthus fraternus were studied. Hypocotyl explants were cultured on B5 medium supplemented with 2,4-D or NAA in the presence and absence of BAP (at concentrations 0, 10(-7), 10(-6) and 10(-5)M). Adventitious shoots differentiated from callus developed from the cut ends of 12.5% of the hypocotyl segments cultured on medium supplemented with 10(-6)M BAP in combination with 10(-6)M 2,4-D or 10(-6)M NAA. Profuse rooting occurred from the hypocotyl explants on medium supplemented with 10(-6)M BAP + 10(-6)M NAA. Incorporation of casein hydrolysate in B5 medium along with 10(-6)M BAP + 10(-7)M 2,4-D enhanced the frequency of cultures with adventitious shoots upto 68.0%. Glutamine, glutamic acid or proline could partially substitute for the effect of casein hydrolysate. Amongst the hypocotyls from 3-14 d old seedlings, the best caulogenesis was obtained with hypocotyls from 7 d old seedlings both in presence or absence of casein hydrolysate. Best rooting of shoots was achieved on half-strength B5 medium supplemented with 10(-6)M IBA. After hardening, plantlets were successfully transferred to the soil.
研究了促进叶下珠胚轴外植体产生不定芽的因素。将胚轴外植体在 B5 培养基上培养，该培养基添加了 2,4-D 或 NAA，存在或不存在 BAP（浓度为 0、10(-7)、10(-6)和 10(-5)M）。在添加 10(-6)M BAP 与 10(-6)M 2,4-D 或 10(-6)M NAA 的培养基上培养的胚轴段中，有 12.5%的外植体从切口处的愈伤组织中分化出不定芽。在添加 10(-6)M BAP+10(-6)M NAA 的培养基上，胚轴外植体大量生根。在 B5 培养基中添加水解酪蛋白，同时添加 10(-6)M BAP+10(-7)M 2,4-D，可以将具有不定芽的培养物的频率提高到 68.0%。谷氨酰胺、谷氨酸或脯氨酸可以部分替代水解酪蛋白的作用。在 3-14 天龄的幼苗的胚轴中，在有或没有水解酪蛋白的情况下，7 天龄的幼苗的胚轴产生不定芽的效果最好。在添加 10(-6)M IBA 的半强度 B5 培养基上，芽的生根效果最好。经过硬化后，将幼苗成功移植到土壤中。
