Laboratoire de Physiologie Végétale, Département de Botanique, Université de Liège, Sart Tilman, B-4000, Liège, Belgium.
Planta. 1990 Jun;181(3):324-6. doi: 10.1007/BF00195883.
Whilst the cytokinins are important promoters of plant cell division in vitro and in vivo, their mode of action remains unknown. Here we report the results of a study showing that a single application of a low dose of a cytokinin to the shoot apical meristem of Sinapis alba L. activates new replicon origins in chromosomal DNA, resulting in the halving of replicon size, and synchronizes the activation of replicon origins. These effects cause a 3.5-fold shortening of the duration of chromosomal DNA replication (S phase of the cell cycle). We hypothesize that one of the proteins involved in the initiation of DNA replication is a target for cytokinins.
虽然细胞分裂素在植物体外和体内都是重要的细胞分裂促进剂，但它们的作用模式仍不清楚。在这里，我们报告了一项研究的结果，该研究表明，向白芥（Sinapis alba L.）茎尖分生组织施加低剂量的细胞分裂素可激活染色体 DNA 中的新复制原点，导致复制子大小减半，并使复制原点的激活同步化。这些效应导致染色体 DNA 复制（细胞周期的 S 期）持续时间缩短了 3.5 倍。我们假设参与 DNA 复制起始的蛋白质之一是细胞分裂素的靶标。
