钙通道药物和光/暗对蚕豆保卫细胞中（86）Rb (+) 流动的影响。

Effects of light/dark and calcium-channel drugs on fluxes of (86)Rb (+) in "isolated" guard cells of Vicia faba L.

机构信息

Botany School, University of Cambridge, CB2 3EA, Cambridge, UK.

出版信息

Planta. 1990 Jun;181(3):440-7. doi: 10.1007/BF00195899.

DOI:10.1007/BF00195899

PMID:24196823

Abstract

Experiments on (86)Rb(+) fluxes were used to identify the changes in ionic state induced by transferring stomata from light to darkness. Guard-cell fluxes and contents were measured on "isolated" epidermal strips from Vicia faba L. in which all cells other than the guard cells had been killed by ultrasonic disruption. Closure of stomata in response to darkness was achieved by a large, transient stimulation of (86)Rb(+) efflux from the guard cells, combined with a reduction of ion transfer from cytoplasm to vacuole, but there was little significant change in influx. Removal of blue but not red light appeared to trigger the flux responses associated with darkening. Attempts to inhibit the closing response (using methoxyverapamil, nifedipine and bepridil, compounds possessing activity against the calcium channels of animal cells) were mostly unsuccessful and the significance of this result is discussed.

摘要

实验表明，（86）Rb（+）流的变化可以用来识别气孔由光照到黑暗条件下离子状态的变化。通过超声波破坏去除表皮细胞中的其它细胞（除保卫细胞外），从蚕豆中分离得到“孤立”的表皮条带，以此来测量保卫细胞的离子流和内容物。气孔对黑暗的反应是通过保卫细胞中（86）Rb（+）外流的短暂、大幅刺激来实现的，同时还伴随着细胞质向液泡中离子转移的减少，但流入没有明显变化。去除蓝光而不是红光似乎可以触发与变暗相关的离子流反应。抑制关闭反应的尝试（使用具有抗动物细胞钙通道活性的甲氧维拉帕米、硝苯地平和贝普地尔等化合物）大多没有成功，并且讨论了这一结果的意义。