Laboratoire de Biologie Cellulaire, Institut National de la Recherche Agronomique, Route de Saint Cyr, 78026, Versailles Cedex, France.
Plant Cell Rep. 1993 Jan;12(3):129-32. doi: 10.1007/BF00239092.
Twenty-three independent kanamycin resistant lines were obtained after cocultivation of longterm embryogenic cultures of three Asparagus officinalis L. genotypes with an Agrobacterium tumefaciens strain harboring ß-glucuronidase and neomycin phosphotransferase II genes. All the lines showed ß-glucuronidase activity by histological staining. DNA analysis by Southern blots of the kanamycin resistant embryogenic lines and of a plant regenerated from one of them confirmed the integration of the T-DNA.
经农杆菌介导，将β-葡萄糖醛酸酶和新霉素磷酸转移酶Ⅱ基因导入 3 种芦笋长期胚性培养物后，共获得 23 株卡那霉素抗性的独立株系。所有株系经组织化学染色均显示β-葡萄糖醛酸酶活性。通过 Southern 印迹分析卡那霉素抗性胚性系和其中一株再生植株的 DNA，证实了 T-DNA 的整合。
