Laboratorium voor Genetische Virologie, Vrije Universiteit Brussel, B-1640 St.-Genesius-Rode,FRG.
EMBO J. 1984 Dec 20;3(13):3039-41. doi: 10.1002/j.1460-2075.1984.tb02254.x.
Cultured stem fragments from the monocotyledonous plant Asparagus officinalis infected by the oncogenic bacterium Agrobacterium tumefaciens developed tumorous proliferations. This tissue was propagated in vitro on hormone-free culture medium. The T-DNA-encoded markers nopaline and agrocinopine were unambiguously detected in these tissues. The data demonstrate that stable T-DNA transfer as well as expression of T-DNA genes is possible in at least some monocotyledonous plants. This opens new possibilities for plant genetic engineering using the Ti plasmid as a gene vector.
从被致癌细菌根瘤农杆菌感染的单子叶植物天门冬属的培养茎段中，发育出了肿瘤样增生。这块组织在无激素的培养基中进行了体外繁殖。在这些组织中明确检测到了 T-DNA 编码的胭脂碱和农杆菌素标记物。这些数据表明，至少在一些单子叶植物中，稳定的 T-DNA 转移和 T-DNA 基因的表达是可能的。这为使用 Ti 质粒作为基因载体进行植物遗传工程开辟了新的可能性。
