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油棕(Elaeis guineensis Jacq.)体细胞胚的冷冻保存涉及干燥步骤。

Cryopreservation of oil palm (Elaeis guineensis Jacq.) somatic embryos involving a desiccation step.

机构信息

Laboratoire de Ressources Génétiques et Amélioration des Plantes Tropicales, ORSTOM, 911, av. Agropolis, B.P. 5045, 34032, 01, Montpellier Cedex, France.

出版信息

Plant Cell Rep. 1993 Apr;12(6):352-5. doi: 10.1007/BF00237434.

Abstract

The standard cryopreservation process previously developed for oil palm clones using shiny white, finger-like somatic embryos could be applied in some cases to standard cultures. Its efficiency was markedly improved by completing the 7-day pregrowth period on 0.75 M sucrose by an additional dehydration period carried out either by placing the embryos in the air current of the laminar flow cabinet or in an air tight box containing silica gel. This improved process was successfully applied to 7 different clones. It will facilitate the routine uof cryopreservation for oil palm cultures.

摘要

之前为油棕克隆体开发的标准冷冻保存过程,使用光亮的白色、手指状的体细胞胚胎,可以在某些情况下应用于标准培养物。通过在 0.75 M 蔗糖上完成 7 天的预生长期,然后进行额外的脱水期,将胚胎置于层流培养箱的气流中或置于含有硅胶的密封盒中,显著提高了其效率。这种改进后的方法成功应用于 7 个不同的克隆体。它将促进油棕培养物的常规冷冻保存。

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