Dept. of Chemistry, New York University, 10003, New York, N.Y., USA.
Mol Biol Rep. 1973 Aug;1(2):123-7. doi: 10.1007/BF00357592.
The reaction of polyuridylic acid with sodium bisulfite produces modified polymers in which up to 95% of the uracil residues have been converted to uracil-6-sulfonate residues. A 91.6% bisulfite-saturated polymer was found to resist hydrolysis by spleen phosphodiesterase and phosphorolysis by polynucleotide phosphorylase. Digestion by pancreatic ribonuclease was successful and gave the bisulfite adduct of uridine-3'-phosphate. Treatment of this nucleotide adduct with acid phosphatase afforded the bisulfite adduct of uridine. The ability of polyuridylic acid to bind to ribosomes, and to stimulate the binding of phenylalanine tRNA to ribosomes was abolished by progressive bisulfite saturation of the polymer. The rate of decline of these functionsf with increasing bisulfite content, was less sharp than the loss of phenyl-alanine coding ability o, the modified polymer.
聚尿嘧啶酸与亚硫酸氢钠反应会产生修饰聚合物，其中多达 95%的尿嘧啶残基已转化为尿嘧啶-6-磺酸酯残基。发现 91.6%的亚硫酸氢盐饱和聚合物能够抵抗脾磷酸二酯酶的水解和多核苷酸磷酸化酶的磷酸解。胰腺核糖核酸酶的消化是成功的，并得到了尿苷 3'-磷酸的亚硫酸氢盐加合物。用酸性磷酸酶处理该核苷酸加合物，得到尿苷的亚硫酸氢盐加合物。聚尿嘧啶酸结合核糖体的能力，并刺激苯丙氨酸 tRNA 与核糖体结合的能力，随着聚合物中亚硫酸氢盐饱和度的增加而被破坏。这些功能的下降速度与修饰聚合物中苯丙氨酸编码能力的丧失相比，下降速度不太陡峭。
