用于模式生物基因组的 CRISPR/Cas9 系统的向导 RNA 序列设计平台。
A guide RNA sequence design platform for the CRISPR/Cas9 system for model organism genomes.
机构信息
Biomedical Engineering Department, College of Engineering, Peking University, Beijing 100871, China.
出版信息
Biomed Res Int. 2013;2013:270805. doi: 10.1155/2013/270805. Epub 2013 Oct 3.
Cas9/CRISPR has been reported to efficiently induce targeted gene disruption and homologous recombination in both prokaryotic and eukaryotic cells. Thus, we developed a Guide RNA Sequence Design Platform for the Cas9/CRISPR silencing system for model organisms. The platform is easy to use for gRNA design with input query sequences. It finds potential targets by PAM and ranks them according to factors including uniqueness, SNP, RNA secondary structure, and AT content. The platform allows users to upload and share their experimental results. In addition, most guide RNA sequences from published papers have been put into our database.
Cas9/CRISPR 已被报道能够在原核细胞和真核细胞中有效诱导靶向基因敲除和同源重组。因此，我们开发了一个 Cas9/CRISPR 沉默系统用于模式生物的 Guide RNA 序列设计平台。该平台使用输入查询序列进行 gRNA 设计非常方便。它通过 PAM 寻找潜在的靶标，并根据包括独特性、SNP、RNA 二级结构和 AT 含量在内的因素对它们进行排序。该平台允许用户上传和共享他们的实验结果。此外，已将大多数来自已发表论文的 Guide RNA 序列放入我们的数据库中。
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