Aluko B T, Oloyede O I, Afolayan A J
Department of Biochemistry, University of Ado Ekiti, Nigeria.
Pak J Biol Sci. 2013 Jan 1;16(1):22-30. doi: 10.3923/pjbs.2013.22.30.
This study assessed the polyphenolic contents and antioxidant activity of ethanol, butanol and ethyl acetate extracts of Ocimum americanum leaves using in vitro models. The ability of the extracts to scavenge 2,2-diphenyl-1-picryihydrazyl (DPPH*), 2,2-azino-bis-(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS*+), hydrogen peroxide (H2O2), Nitric Oxide (NO) and hydroxyl radical (OH*) was investigated. The inhibition of lipid oxidation, reducing power, total flavonoids, phenols and flavonols contents of the extracts were also determined using spectrophotometric methods. The result revealed highest concentration of polyphenolic compounds in the ethanol extract followed by n-butanol while ethyl acetate extract contained the least concentration. Free radical scavenging potentials of the extracts were found to be proportional to their respective polyphenolic contents. This study provides evidence that O. americanum leaves contain biologically active components with effective antioxidant activity and thus could be used for the management of radical related diseases.
本研究采用体外模型评估了美国罗勒叶乙醇提取物、丁醇提取物和乙酸乙酯提取物中的多酚含量及抗氧化活性。研究了这些提取物清除2,2-二苯基-1-苦基肼自由基（DPPH*）、2,2-联氮-双-（3-乙基苯并噻唑啉-6-磺酸）自由基（ABTS*+）、过氧化氢（H2O2）、一氧化氮（NO）和羟自由基（OH*）的能力。还采用分光光度法测定了提取物对脂质氧化的抑制作用、还原能力、总黄酮、酚类和黄酮醇含量。结果显示，乙醇提取物中多酚化合物的浓度最高，其次是正丁醇，而乙酸乙酯提取物中多酚化合物的浓度最低。发现提取物的自由基清除能力与其各自的多酚含量成正比。本研究提供了证据表明美国罗勒叶含有具有有效抗氧化活性的生物活性成分，因此可用于治疗与自由基相关的疾病。
