Igwilo I O, Ogoke T J, Ogbu D O, Igwilo S N, Obi E, Abdulsalami M S
Department of Applied Biochemistry, Nnamdi Azikiwe University, Awka, Nigeria.
Pak J Biol Sci. 2013 Jan 1;16(1):48-50. doi: 10.3923/pjbs.2013.48.50.
Moringa oleifera is a multipurpose tree, cultivated in the tropics and sub-tropics for its nutritional and therapeutic properties. The raw matured seeds which have been reported to be used as food and clarifying agent of turbid water caused growth retardation in albino rats and this might have been due to its antinutritional contents. Owing to these adverse factors, the effect of soaking the seeds for 30 min and then compounding it as feeds was done. Its effect on growth rate and the level of some biochemical parameters on rats were investigated. The Wistar albino rats were fed for 21 days and their weights measured at 2 days interval. Aspartate and Alanine transaminases, Alkaline phosphatase and total bilirubin levels were assayed using Automated Vitros 350 Chemistry Analyzer. The growth rates of rats fed with the commercial rat pellets, Casilan diet and the processed Moringa seed diet had a range of 80.06 +/- 3.54 to 100.98 +/- 5.37, 66.70 +/- 7.54 to 55.23 +/- 7.47 and 52.99 +/- 4.15 to 35.47 +/- 2.26, respectively. The parameters assayed for the group that received the processed Moringa seed diet are 144.00 +/- 16.80 (AST), 41.00 +/- 7.05 (ALT), 66.50 +/- 8.80 (ALP) and 12.45 +/- 1.18 (Total Bilirubin). The one-way ANOVA statistical analysis indicated that there was no significant change in the parameters of all the groups at 95% significance level (p < 0.05). Hence, the soaked Moringa oleifera seed did not support growth in the animal subjects and also did not pose a threat to the liver. However, it is better to develop more suitable processing methods to improve the seed's nutritional capabilities.
辣木是一种多用途树木，因其营养和治疗特性而在热带和亚热带地区种植。据报道，未加工的成熟种子被用作食物和浑浊水的澄清剂，这导致白化大鼠生长迟缓，这可能是由于其抗营养成分所致。由于这些不利因素，进行了将种子浸泡30分钟然后将其配制成饲料的实验。研究了其对大鼠生长速率和一些生化参数水平的影响。用Wistar白化大鼠喂养21天，并每隔2天测量其体重。使用自动维特思350化学分析仪测定天冬氨酸和丙氨酸转氨酶、碱性磷酸酶和总胆红素水平。喂食商业鼠粮、酪蛋白饮食和加工后的辣木籽饮食的大鼠生长速率范围分别为80.06±3.54至100.98±5.37、66.70±7.54至55.23±7.47和52.99±4.15至35.47±2.26。接受加工后的辣木籽饮食组的检测参数分别为144.00±16.80（天冬氨酸转氨酶）、41.00±7.05（丙氨酸转氨酶）、66.50±8.80（碱性磷酸酶）和12.45±1.18（总胆红素）。单因素方差分析统计表明，在95%显著性水平（p<0.05）下，所有组的参数均无显著变化。因此，浸泡后的辣木籽不支持动物生长，对肝脏也没有威胁。然而，最好开发更合适的加工方法来提高种子的营养能力。
