灯盏花黄酮合酶II基因的克隆及生物信息学分析
[Cloning and bioinformation analysis of flavone synthase II gene of Erigeron breviscapus].
作者信息
Zhang Guang-Hui, Li Cui-Ting, Wang Jian-Jun, Zhang Wei, Chen Jun-Wen, Yang Jian-Wen, Yang Sheng-Chao
机构信息
Yunnan Research Center on Good Agricultural Practice for Dominant Chinese Medicinal Materials, Yunnan Agricultural University, Kunming 650201, China.
出版信息
Zhongguo Zhong Yao Za Zhi. 2013 Jul;38(14):2231-6.
OBJECTIVE
Erigeron breviscapus is a medicinal plant with the most developmental potential in Yunnan province, which is belongs to Erigeron genus of Compositae family. Scutellarin, the main active component of Erigeron breviscapus is one of flavone 7-O-glucuronide derivatives, its biosynthesis pathway is still not clear.
METHOD
Full length cDNA encoding flavone syhthase II gene in E. breviscapus was cloned in this study using R-PCR, 3'-RACE and 5'-RACE.
RESULT
The opening reading frame of FS II cDNA of E. breviscapus is 1 557 bp long and encoding 518 amino acids, designed as EbFS II, which is highly homologous with FS II of Compositae species, like Callistephus chinensis, Cynara cardunculus var. scolymus, Gerbera hybrida, Dahlia pinnata and Lobelia erinus.
CONCLUSION
Phylogenetic analysis showed that EbFS II might has the function of directly converting flavanone to flavone.
目的
灯盏花是云南省最具开发潜力的药用植物，属于菊科飞蓬属。灯盏花的主要活性成分灯盏乙素是黄酮7 - O - 葡萄糖醛酸苷衍生物之一，其生物合成途径尚不清楚。
方法
本研究采用R - PCR、3'-RACE和5'-RACE技术克隆了灯盏花中编码黄酮合酶II基因的全长cDNA。
结果
灯盏花FS II cDNA的开放阅读框长1557 bp，编码518个氨基酸，命名为EbFS II，它与菊科植物如翠菊、菜蓟、非洲菊、大丽花和半边莲的FS II高度同源。
结论
系统发育分析表明，EbFS II可能具有将黄烷酮直接转化为黄酮的功能。
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