Zhang Wei, Wang Jian-Jun, Wei Xiang, Zhang Guang-Hui, Yang Jian-Wen, Wu Dao-Cong, Yang Sheng-Chao
Yunnan Research Center on Good Agriculture Practice for Dominant Chinese Medicinal Materials, Yunnan Agriculture University, Kunming 650201, China.
Zhongguo Zhong Yao Za Zhi. 2013 Jul;38(14):2245-9.
To analyze the genetic diversity and breeding strains of the E. breviscapus germplasms, in order to provide theoretical information for Erigeron breviscapus breeding.
The genetic diversity and genetic structure were assayed to six germplasm resource of E. breviscapus which collected from Yunnna with 11 pairs primers and AFLP molecular marker.
Six hundred and four amplification bands among 636 DNA bands were from six accession of E. breviscapus, which are about 82.40% of total bands. The six germplasms could be divided into three group at the 0. 706 similarity coefficient level. The first category include QS-1, QS-2 and Dali, Shilin, Kunming population. The second category included wild population of Qiubei. The third category included several sample from different district. The mean genetic similarity coefficient of QS-1 and QS-2 was bigger, genetic similarity coefficient range was smaller, hereditary character was more stable. Molecular system clustering analysis showed that the geographical origin of the same part had relative polymerization phenomenon and its genetic relationship was close. Qiubei was a single group possibly relating to the specific genetic basis.
The analysis of genetic diversity of E. breviscapus by AFLP marker is reliable. The systematic E. breviscapus breeding is feasible.
分析灯盏花种质资源的遗传多样性和育种品系，为灯盏花育种提供理论依据。
采用AFLP分子标记技术，利用11对引物对从云南收集的6份灯盏花种质资源进行遗传多样性和遗传结构分析。
6份灯盏花种质在636条DNA条带中扩增出604条带，占总条带数的82.40%。在相似系数为0.706水平上，6份种质可分为3类。第一类包括QS-1、QS-2以及大理、石林、昆明群体；第二类包括丘北野生群体；第三类包括来自不同地区的多个样本。QS-1与QS-2的平均遗传相似系数较大，遗传相似系数范围较小，遗传性状较稳定。分子系统聚类分析表明，同一产地的材料有相对聚合现象，亲缘关系较近。丘北为单一类群，可能具有特定的遗传基础。
利用AFLP标记分析灯盏花遗传多样性结果可靠，灯盏花系统育种可行。
