[高效液相色谱法同时测定灯盏细辛不同部位绿原酸、野黄芩苷、3,5-二咖啡酰奎宁酸、4,5-二咖啡酰奎宁酸的含量]

[Simultaneous determination of chlorogenic acid, scutellarin, 3,5-dicaffeoylquinic acid, 4,5-dicaffeoylquinic acid in different parts of Erigeron breviscapus by high-performance liquid chromatography].

作者信息

Li Xiao-Bo, Wang Rui-Bo, Shen Yong, Meng Zhen-Gui, Chen Jun-Wen, Yang Jian-Wen, Yang Sheng-Chao

机构信息

Yunnan Research Center on Good Agriculture Practice for Dominant Chinese Medicinal Meterials, Yunnan Agriculture University, Kunming 650201, China.

出版信息

Zhongguo Zhong Yao Za Zhi. 2013 Jul;38(14):2237-40.

PMID:24199546

Abstract

OBJECTIVE

A high-performance liquid chromatographic (HPLC) method was developed for simultaneous determination of chlorogenic acid, scutellarin, 3,5-dicaffeoylquinic acid, 4,5-dicaffeoylquinic acid in different parts of Erigerontis Herba.

METHOD

The four constituents were measured on an Agilent Zorbax SB-C18 column (4.6 mm x 450 mm, 5 microm) with a gradient elution of acetonitrile (A) -0.3% phosphoric acid solution (B) (0-10 min, 12%-15% A, 10-32 min, 15% A, 32-33 min, 15%-20% A, 33-50 min, 20%-22% A) at wavelength of 335 nm and 327 nm, and a flow rate of 1.0 mL x min(-1) and the column temperature was 30 degrees C.

RESULT

Linearity of each standard was established in the concentration range of 0.050 1-1.002 microg for chlorogenic acid, 0.165 9-3.318 microg for chlorogenic acid, 0.049 7-0.994 microg for 3,5-dicaffeoylquinic acid, 0.048 7-0.974 p.g for 4,5-dicaffeoylquinic acid respectively, with correlation coefficient r > 0.999 6. Average recoveries (n = 6) of 4 compounds were 98.53% with a RSD of 0.94%, 99.68% with a RSD of 0.49%, 98.78% with a RSD of 1.1%, 99.06% with a RSD of 0.81%, respectively.

CONCLUSION

The developed method is simple, accurate, and precise, it can be used for the quantitative analysis of Erigeron breviscapus.

摘要

目的

建立高效液相色谱法同时测定灯盏细辛不同部位中绿原酸、灯盏花素、3,5 - 二咖啡酰奎宁酸、4,5 - 二咖啡酰奎宁酸的含量。

方法

采用Agilent Zorbax SB - C18柱（4.6 mm×450 mm，5 µm），以乙腈（A）-0.3%磷酸溶液（B）为流动相进行梯度洗脱（0 - 10 min，12% - 15% A；10 - 32 min，15% A；32 - 33 min，15% - 20% A；33 - 50 min，20% - 22% A），检测波长为335 nm和327 nm，流速为1.0 mL·min⁻¹，柱温为30℃。

结果

绿原酸在0.050 1 - 1.002 μg浓度范围内、灯盏花素在0.165 9 - 3.318 μg浓度范围内、3,5 - 二咖啡酰奎宁酸在0.049 7 - 0.994 μg浓度范围内、4,5 - 二咖啡酰奎宁酸在0.048 7 - 0.974 μg浓度范围内线性关系良好，相关系数r＞0.999 6。4种化合物的平均回收率（n = 6）分别为98.53%，RSD为0.94%；99.68%，RSD为0.49%；98.78%，RSD为1.1%；99.06%，RSD为0.81%。