高效液相色谱等度洗脱法测定银黄薄膜衣片中绿原酸和黄芩苷的含量
[Determination of chlorogenic acid and baicalin in Yinhuang film-coated tablets by HPLC isocratic elution].
作者信息
Xu Mei, Yuan Qi, Zhao Hui, Li Fan, Hu Xiao-Lei, Fu Ying-Ying, Shi Guo-Hua, Wang Xiao-Ming, Zhang Na
机构信息
Department of Pharmaceutical Analysis, College of Pharmacy, Henan University, Kaifeng 475001, China.
出版信息
Zhongguo Zhong Yao Za Zhi. 2013 Mar;38(5):691-3.
OBJECTIVE
To establish an HPLC method for determination of chlorogenic acid and baicalin in Yinhuang film-coated tablets.
METHOD
HPLC isocratic elution was adopted, with the C18 column (4.6 mm x 250 mm, 5 microm) as the chromatographic column, methanol-water-phosphoric acid (45: 55: 0.2) as the mobile phase. The flow rate was set at 1.0 mL x min(-1), and column temperature was set at 30 degrees C. The detection wavelength was 328 nm, and the sample size was 20 microL.
RESULT
At concentrations ranging between 2.200-44.00, 24.20-242.0 mg x L(-1), peak area of chlorogenic acid and baicalin showed good linear relationship, the average recoveries were 99.63% (RSD 1.8%, n = 9) and 99.72% (RSD 1.9%, n = 9), respectively.
CONCLUSION
The method is simple that it can accurately measure the content of chlorogenic acid and baicalin in Yinhuang film-coated tablets.
目的
建立一种测定银黄薄膜衣片中绿原酸和黄芩苷含量的高效液相色谱法。
方法
采用高效液相色谱等度洗脱，以C18柱（4.6 mm×250 mm，5 µm）为色谱柱，甲醇 - 水 - 磷酸（45∶55∶0.2）为流动相。流速设定为1.0 mL·min⁻¹，柱温设定为30℃。检测波长为328 nm，进样量为20 µL。
结果
绿原酸和黄芩苷在2.200 - 44.00、24.20 - 242.0 mg·L⁻¹浓度范围内峰面积呈良好线性关系，平均回收率分别为99.63%（RSD 1.8%，n = 9）和99.72%（RSD 1.9%，n = 9）。
结论
该方法简便，可准确测定银黄薄膜衣片中绿原酸和黄芩苷的含量。
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