Qin Yueqiu, Liao Pinhu, He Shougao, Yin Yixia, Song Sien, Hu Jing, Zheng Pin
Department of Digestive Medicine, Affiliated Hospital, Youjiang Medical College for Nationalities, Baise 533000, China.
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2013 Nov;29(11):1189-92.
To investigate the levels of FasL mRNA in peripheral blood mononualear cells (PBMCs), serum soluble Fas ligand (sFasL) and their regulatory effect on T lymphocyte subsets in patients with severe acute pancreatitis (SAP).
Forty-eight patients with pancreatitis were randomly divided into two groups: 20 cases with SAP and 28 cases with mild acute pancreatitis (MAP). Twenty-eight healthy volunteers were selected as control group. The expression of FasL mRNA in PBMCs was detected by real-time quantitative PCR(qRT-PCR), and serum sFasL was measured by ELISA. T lymphocyte subsets in peripheral blood were detected by flow cytometry.
Compared with control group and MAP group, FasL mRNA of PBMCs and serum sFasL increased significantly in SAP group (P<0.05), a little increase in MAP group, and there was no significant difference between MAP group and control group (P>0.05). The CD4(+) T cell ratio, CD4(+)/CD8(+) ratio decreased significantly in SAP group (P<0.05) vs control group and MAP group), and they were found negatively related to FasL mRNA, serum sFasL level.
The SAP patients showed the significantly increased FasL mRNA of PBMCs and serum sFasL and decreased CD4(+) T-cell ratio, CD4(+)/CD8(+) ratio. FasL may mediate the apoptosis of T lymphocytes.
探讨重症急性胰腺炎（SAP）患者外周血单个核细胞（PBMCs）中FasL mRNA水平、血清可溶性Fas配体（sFasL）水平及其对T淋巴细胞亚群的调节作用。
将48例胰腺炎患者随机分为两组：20例SAP患者和28例轻症急性胰腺炎（MAP）患者。选取28名健康志愿者作为对照组。采用实时定量聚合酶链反应（qRT-PCR）检测PBMCs中FasL mRNA的表达，采用酶联免疫吸附测定（ELISA）法检测血清sFasL。采用流式细胞术检测外周血T淋巴细胞亚群。
与对照组和MAP组相比，SAP组PBMCs中FasL mRNA和血清sFasL显著升高（P<0.05），MAP组略有升高，MAP组与对照组之间无显著差异（P>0.05）。与对照组和MAP组相比，SAP组CD4(+)T细胞比例、CD4(+)/CD8(+)比例显著降低（P<0.05），且与FasL mRNA、血清sFasL水平呈负相关。
SAP患者PBMCs中FasL mRNA和血清sFasL显著升高，CD4(+)T细胞比例、CD4(+)/CD8(+)比例降低。FasL可能介导T淋巴细胞凋亡。
