Pinhu Liao, Qin Yueqiu, Xiong Bin, You Yanwu, Li Jun, Sooranna Suren R
Intensive Care Unit, Affiliated Hospital of Youjiang Medical University for Nationalities, No. 18 Zhongshan Road II, 533000, Baise, Guangxi Zhuang Autonomous Region, China,
Inflammation. 2014 Aug;37(4):1202-12. doi: 10.1007/s10753-014-9847-8.
This study investigated the relationship of Fas and Fas ligand (FasL) expression and apoptosis of lymphocytes in relation to the pathogenic immune response and infectious complications observed in experimental severe acute pancreatitis in mice. Forty male Balb/c mice were randomly divided into control, mild (MAP), and severe acute pancreatitis (SAP) groups. Overexpression of Fas/FasL messenger ribonucleic acid (mRNA) and protein was observed in spleen-derived lymphocytes in SAP (p < 0.01). Apoptosis of these resulted in a depletion of circulating lymphocytes in this group (p < 0.05). A further significant change in the SAP group with infectious complications was observed. A positive relationship was found between the Fas/FasL expression and lymphocyte apoptosis, and negative relationships were observed between Fas/FasL expression and CD4(+) and CD19(+) lymphocytes and the CD4(+)/CD8(+) ratio in SAP mice (p < 0.01). The results suggest that the overexpression of Fas/FasL is associated with infectious complications and severity of experimental severe acute pancreatitis by promoting apoptosis of lymphocytes.
本研究调查了Fas和Fas配体（FasL）的表达与淋巴细胞凋亡之间的关系，以及它们与在实验性重症急性胰腺炎小鼠中观察到的致病性免疫反应和感染性并发症的关系。40只雄性Balb/c小鼠被随机分为对照组、轻症（MAP）组和重症急性胰腺炎（SAP）组。在SAP组的脾源性淋巴细胞中观察到Fas/FasL信使核糖核酸（mRNA）和蛋白的过表达（p < 0.01）。这些细胞的凋亡导致该组循环淋巴细胞减少（p < 0.05）。在伴有感染性并发症的SAP组中观察到进一步的显著变化。在SAP小鼠中，发现Fas/FasL表达与淋巴细胞凋亡呈正相关，而Fas/FasL表达与CD4(+)和CD19(+)淋巴细胞以及CD4(+)/CD8(+)比值呈负相关（p < 0.01）。结果表明，Fas/FasL的过表达通过促进淋巴细胞凋亡与实验性重症急性胰腺炎的感染性并发症和严重程度相关。
