Health and Exercise Sciences Research Group, University of Stirling, Stirling FK9 4LA, United Kingdom.
Health and Exercise Sciences Research Group, University of Stirling, Stirling FK9 4LA, United Kingdom; Laboratory of Integrated Physiology, Department of Health and Human Performance, University of Houston, Houston, TX, USA.
Brain Behav Immun. 2014 Jul;39:152-9. doi: 10.1016/j.bbi.2013.10.031. Epub 2013 Nov 4.
Investigate training status and sex effects on the redistribution of senescent and naïve T-lymphocytes following acute exercise.
Sixteen (8 male, 8 female) trained (18.3±1.7yr) soccer players (Tr) and sixteen (8 male, 8 female) untrained (19.3±2.0yr) controls (UTr) performed a treadmill running test to volitional exhaustion. Blood lymphocytes were isolated before (Pre), immediately post, and 1-h post-exercise for assessment of cell surface expression of CD28 and CD57 on CD4(+) and CD8(+) T-lymphocyte subsets. Plasma was used to determine cytomegalovirus (CMV) serostatus.
Exercise elicited a redistribution of T-lymphocyte subsets. Senescent CD4(+) and CD8(+) T-lymphocytes increased by 42.4% and 45.9% respectively, while naïve CD4(+) and CD8(+) T-lymphocytes decreased by 8.7% and 22.5% respectively in response to exercise. A main effect (P<0.05) of training status was observed for senescent CD4(+), CD8(+) and naïve CD8(+) T-lymphocytes: UTr had a higher proportion of senescent and a lower proportion of naïve CD8(+) T-lymphocytes than Tr. A main effect (P<0.05) of sex was observed in senescent CD4(+), CD8(+) and naïve CD4(+), CD8(+) T-lymphocytes. Males had a higher proportion of senescent and lower proportion of naïve T-lymphocytes than females. A sex-by-training status interaction (P<0.05) was observed for the senescent and naïve CD4(+) T-lymphocytes (but not CD8(+)) with the highest percentage of senescent and lowest percentage of naïve T-lymphocytes observed in UTr males. CMV exerted a significant main covariate effect (P<0.05) in the senescent and naïve (P<0.05) CD8(+) T-lymphocytes but not in the senescent and naïve CD4(+) T-lymphocytes.
This study highlights important sex and training status differences in the senescent and naïve T-lymphocyte redistribution in response to exercise that warrants further investigation.
研究训练状态和性别对急性运动后衰老和幼稚 T 淋巴细胞再分布的影响。
16 名（男 8 名，女 8 名）训练有素的（18.3±1.7 岁）足球运动员（Tr）和 16 名（男 8 名，女 8 名）未经训练的（19.3±2.0 岁）对照组（UTr）进行跑步机跑步测试至力竭。在运动前（Pre）、运动后即刻和运动后 1 小时分离血液淋巴细胞，用于评估 CD4(+)和 CD8(+)T 淋巴细胞亚群上 CD28 和 CD57 的细胞表面表达。使用血浆来确定巨细胞病毒（CMV）血清状态。
运动引起 T 淋巴细胞亚群的再分布。衰老的 CD4(+)和 CD8(+)T 淋巴细胞分别增加了 42.4%和 45.9%，而幼稚的 CD4(+)和 CD8(+)T 淋巴细胞分别减少了 8.7%和 22.5%。训练状态的主要效应（P<0.05）观察到衰老的 CD4(+)、CD8(+)和幼稚的 CD8(+)T 淋巴细胞：UTr 中衰老的 CD8(+)T 淋巴细胞比例较高，幼稚的 CD8(+)T 淋巴细胞比例较低。性别的主要效应（P<0.05）观察到衰老的 CD4(+)、CD8(+)和幼稚的 CD4(+)、CD8(+)T 淋巴细胞。男性衰老的 CD4(+)和 CD8(+)T 淋巴细胞比例较高，幼稚的 CD4(+)和 CD8(+)T 淋巴细胞比例较低。衰老和幼稚的 CD4(+)T 淋巴细胞（但不是 CD8(+)）观察到 UTr 男性衰老的 CD4(+)T 淋巴细胞比例最高，幼稚的 CD4(+)T 淋巴细胞比例最低，性别与训练状态之间存在显著的交互作用（P<0.05）。巨细胞病毒对衰老和幼稚的（P<0.05）CD8(+)T 淋巴细胞产生显著的主要协变量效应，但对衰老和幼稚的 CD4(+)T 淋巴细胞没有影响。
本研究强调了性别和训练状态对运动后衰老和幼稚 T 淋巴细胞再分布的重要差异，值得进一步研究。
