Pharmacy School, Wenzhou Medical University, Wenzhou City, Zhejiang Province, China; St. Christopher's Hospital for Children/Drexel University College of Medicine, Philadelphia, PA, USA.
Pharmacy Department, Hangzhou Xiasha Hospital, Hangzhou, Zhejiang Province, China.
Nanomedicine. 2014 May;10(4):755-64. doi: 10.1016/j.nano.2013.10.009. Epub 2013 Nov 4.
Lipid nanoparticles with solid matrix have been given increasing attention due to their biodegradable status and ability to entrap a variety of biologically active compounds. In this study, new phospholipid-based gelatin nanoparticles encapsulating basic fibroblast growth factor (bFGF) were developed to target the brain via nasal administration. Treatment effects were assessed by quantifying rotational behavior, monoamine neurotransmitter levels and tyrosine hydroxylase expression in 6-hydroxydopamine induced hemiparkinsonian rats. The gelatin nanostructured lipid carriers (GNLs) were prepared by a water-in-water emulsion method and then freeze-dried. The GNLs possessed better profile than gelatin nanoparticles (GNs), with particle size 143±1.14nm and Zeta potential -38.2±1.2mV. The intranasal GNLs efficiently enriched exogenous bFGF in olfactory bulb and striatum without adverse impact on the integrity of nasal mucosa and showed obvious therapeutic effects on hemiparkinsonian rats. Thus, GNLs are attractive carriers for nose-to-brain drug delivery, especially for unstable macromolecular drugs such as bFGF.
This team of authors reports the development of phospholipid-based gelatin nanoparticles encapsulating basic fibroblast growth factor to target the brain via intranasal administration. A rat model of hemiparkinsonism was applied demonstrating a good safety profile and an obvious therapeutic effect.
由于具有生物可降解性和能够包埋各种生物活性化合物的能力，具有固体基质的脂质纳米颗粒受到了越来越多的关注。在这项研究中，开发了新型基于磷脂的明胶纳米颗粒，通过鼻腔给药将碱性成纤维细胞生长因子（bFGF）包封。通过量化旋转行为、单胺神经递质水平和酪氨酸羟化酶在 6-羟多巴胺诱导的半帕金森大鼠中的表达来评估治疗效果。明胶结构脂质载体（GNLs）通过水包水乳液法制备，然后冷冻干燥。GNLs 比明胶纳米颗粒（GNs）具有更好的性能，粒径为 143±1.14nm，Zeta 电位为-38.2±1.2mV。鼻内 GNLs 能够有效地将外源性 bFGF 富集到嗅球和纹状体中，而不会对鼻黏膜的完整性产生不利影响，并对半帕金森大鼠表现出明显的治疗效果。因此，GNLs 是一种有吸引力的脑内递药载体，特别是对于 bFGF 等不稳定的大分子药物。
本研究小组报告了开发基于磷脂的明胶纳米颗粒，通过鼻腔给药将碱性成纤维细胞生长因子（bFGF）包封以靶向大脑。应用半帕金森大鼠模型证明了良好的安全性和明显的治疗效果。
