Faculty of Forestry and Wood Technology, Mendel University, Zemědělská 3, 613 00 Czech Republic.
Tree Physiol. 2013 Oct;33(10):1099-110. doi: 10.1093/treephys/tpt082. Epub 2013 Nov 6.
The aim of the present study was to evaluate the adenosine triphospate (ATP), glucose-6-phosphate (glu-6P) and reduced form of nicotinamide adenine dinucleotide phosphate (NAD(P)H) cellular levels during the proliferation and maturation phases of Abies alba Mill. somatic embryos. For a better understanding of the dynamics of these parameters during the proliferation cycle, four embryonic cell lines were tested. During the maturation period, three independent experiments were conducted, focused on the effects of PEG-4000 (5 or 10% (w/v)) and abscisic acid (16, 32 or 64 μM) applied together (Experiments A and B) or with addition of gibberellic acid (Experiment C) on the dynamics of bio-energetic molecules and on the mean number of cotyledonary somatic embryos. Our results demonstrated that the cellular levels of bio-energetic molecules strongly depended on the composition of maturation media. Generally, the higher the number of cotyledonary embryos produced, the higher the level of ATP observed after a 2-week maturation period. The cellular level of ATP, glu-6P and NAD(P)H increased, particularly after the transition from the proliferation to the maturation phase when the differentiation and growth of somatic embryos occurred.
本研究旨在评估阿尔卑斯山白云杉体细胞胚胎增殖和成熟阶段的三磷酸腺苷 (ATP)、葡萄糖-6-磷酸 (glu-6P) 和还原型烟酰胺腺嘌呤二核苷酸磷酸 (NAD(P)H) 细胞水平。为了更好地理解这些参数在增殖周期中的动态变化，我们测试了四个胚胎细胞系。在成熟阶段，我们进行了三项独立的实验，重点研究聚乙二醇 4000 (5 或 10%（w/v）) 和脱落酸 (16、32 或 64 μM) 联合应用（实验 A 和 B）或与赤霉素（实验 C）联合应用对生物能量分子动态变化和子叶状体细胞胚平均数量的影响。我们的结果表明，生物能量分子的细胞水平强烈依赖于成熟培养基的组成。通常，在两周的成熟培养后，产生的子叶状胚胎数量越多，观察到的 ATP 水平越高。当体细胞胚胎发生分化和生长时，ATP、glu-6P 和 NAD(P)H 的细胞水平增加，特别是在从增殖到成熟阶段的转变之后。
