School of Allied Health Sciences and Public Health, Walailak University, Tha Sala, Nakhon Si Thammarat 80161, Thailand.
Faculty of Medical Technology, Prince of Songkla University, Hat Yai, Songkhla 90110, Thailand.
J Microbiol Methods. 2014 Jan;96:19-24. doi: 10.1016/j.mimet.2013.10.010. Epub 2013 Nov 5.
The objective of this study was to create a biphasic cultural method for the detection of Helicobacter pylori in gastric biopsy specimens. The biphasic systems were made by using a urea agar slant with overlaying broth in a single vessel. Initially, three different liquid media including brain-heart infusion broth, Brucella broth, and Bolton broth were tested for their ability to support the growth of H. pylori. Bolton broth with 10% defibrinated horse blood demonstrated a significant increase in the numbers of H. pylori (p<0.05). The result showed that positive urease was used to concentrate viable H. pylori cells where the numbers of bacteria were 10(5)cfu. In addition, the reliable incubation time was at least 36h. In total, 55 biopsies were comparatively studied using commercial rapid urease test and PCR. Seven samples (12.72%) were positive with H. pylori by the biphasic test. With the CLOtest, 6 (10.91%) samples were positive. In conclusion, the Hp biphasic test achieved more positive samples than did the commercial rapid urease test.
本研究旨在创建一种双相文化方法，用于检测胃活检标本中的幽门螺杆菌。双相系统是通过在单个容器中使用带有覆盖肉汤的尿素琼脂斜面制成的。最初，测试了三种不同的液体培养基，包括脑心浸液肉汤、布鲁氏菌肉汤和 Bolton 肉汤，以测试它们支持幽门螺杆菌生长的能力。含 10%去纤维马血的 Bolton 肉汤显示出幽门螺杆菌数量的显著增加（p<0.05）。结果表明，阳性尿素酶用于浓缩有活力的幽门螺杆菌细胞，细菌数量为 10(5)cfu。此外，可靠的孵育时间至少为 36 小时。总共比较了 55 个活检标本，使用商业快速尿素酶试验和 PCR。双相试验中，有 7 个样本（12.72%）幽门螺杆菌阳性。CLOtest 检测，有 6 个样本（10.91%）阳性。总之，Hp 双相试验比商业快速尿素酶试验获得了更多的阳性样本。
