Boyanova L, Stancheva I, Todorov D, Kumanova R, Petrov S, Vladimirov B, Pehlivanov N, Mitova R, Chakarski I, Churchev I
Department of Microbiology, Medical University, Sofia, Bulgaria.
Eur J Gastroenterol Hepatol. 1996 Sep;8(9):911-4.
To propose two modified urease tests for the detection of Helicobacter pylori in gastric biopsy specimens.
The presence of H. pylori infection was determined in 237 patients undergoing upper gastrointestinal endoscopy. Three media were used for the urease tests: Christensen's 2% urea broth and two urea agar media, modified by increasing the concentration of urea (to 4% and 10%) and phenol red and omitting the nutrients.
The modified tests had good sensitivity (> 78%), specificity (96%) and accuracy (> or = 86%) at 2 h using small amounts (15%) of biopsy homogenates. They were statistically more sensitive and accurate than Christensen's broth.
Both the modified 4% and 10% urea agar tests are simple, sensitive and specific and can be performed with small amounts of sample.
提出两种改良脲酶试验，用于检测胃活检标本中的幽门螺杆菌。
对237例接受上消化道内镜检查的患者进行幽门螺杆菌感染检测。三种培养基用于脲酶试验：克里斯滕森2%尿素肉汤和两种尿素琼脂培养基，通过提高尿素浓度（至4%和10%）、加入酚红并省略营养成分进行改良。
改良试验在2小时时使用少量（15%）活检匀浆具有良好的敏感性（>78%）、特异性（96%）和准确性（≥86%）。它们在统计学上比克里斯滕森肉汤更敏感、更准确。
改良的4%和10%尿素琼脂试验均简单、敏感且特异，且可用少量样本进行检测。
