Institut de Génétique et Microbiologie, CNRS/UMR 8621, Centre scientifique d'Orsay, Université Paris-Sud, Bâtiment 400, 91405 Orsay Cedex, France; CHRU de Tours, Université François Rabelais de Tours, UMR1282 ISP, F-37032 Tours, France; INRA, UMR1282 ISP, F-37380 Nouzilly, France.
Institut de Génétique et Microbiologie, CNRS/UMR 8621, Centre scientifique d'Orsay, Université Paris-Sud, Bâtiment 400, 91405 Orsay Cedex, France.
J Microbiol Methods. 2014 Jan;96:16-8. doi: 10.1016/j.mimet.2013.10.020. Epub 2013 Nov 5.
Streptococcus agalactiae is the principal cause of neonatal infections; however, for its study, genetic tools are scarce. We have developed pG+off, an expression vector with a tetracycline inducible promoter that allows a tightly-controllable gene expression in S. agalactiae. Using a reporter gene, we have shown that pG+off is characterized by tight repression under non-induced condition.
无乳链球菌是新生儿感染的主要原因；然而，对于其研究，遗传工具却很匮乏。我们开发了 pG+off，这是一种带有四环素诱导启动子的表达载体，可在无乳链球菌中实现严格可控的基因表达。通过报告基因，我们已经表明 pG+off 在非诱导条件下受到严格抑制。
