Nishizawa Keito, Hiraga Susumu, Yasue Hiroshi, Chiba Mitsuru, Tougou Makoto, Nanjo Yohei, Komatsu Setsuko
NARO Institute of Crop Science.
Biosci Biotechnol Biochem. 2013;77(11):2205-9. doi: 10.1271/bbb.130384. Epub 2013 Nov 7.
Cytosolic ascorbate peroxidases (cAPXs) of soybean have been found by proteome analysis to be downregulated in submerged seedlings. To elucidate the physiological meaning of this downregulation, soybean cAPXs were characterized in this study. Vigorous synthesis was detected in germinating seeds and seedlings. Expression of the corresponding genes was detected clearly in tissues that actively underwent cell division. The gene expression was suppressed by flooding stress, but not by salinity, cold or drought stress. The expression recovered 1 d after release from flooding stress, accompanied by growth resurgence.
通过蛋白质组分析发现，大豆的胞质抗坏血酸过氧化物酶（cAPXs）在淹水幼苗中表达下调。为阐明这种下调的生理意义，本研究对大豆cAPXs进行了表征。在发芽的种子和幼苗中检测到旺盛的合成。在积极进行细胞分裂的组织中清晰地检测到了相应基因的表达。该基因表达受到淹水胁迫的抑制，但不受盐胁迫、冷胁迫或干旱胁迫的影响。从淹水胁迫解除1天后，该表达恢复，同时生长复苏。
