Faculty of Environment and Information Sciences, Fukui University of Technology, Fukui 910-8505, Japan.
Department of Plant Sciences, Quaid-i-Azam University, Islamabad 45320, Pakistan.
Int J Mol Sci. 2020 Feb 14;21(4):1300. doi: 10.3390/ijms21041300.
Extensive utilization of silver nanoparticles (NPs) in agricultural products results in their interaction with other chemicals in the environment. To study the combined effects of silver NPs with nicotinic acid and potassium nitrate (KNO), a gel-free/label-free proteomic technique was used. Root length/weight and hypocotyl length/weight of soybean were enhanced by silver NPs mixed with nicotinic acid and KNO. Out of a total 6340 identified proteins, 351 proteins were significantly changed, out of which 247 and 104 proteins increased and decreased, respectively. Differentially changed proteins were predominantly associated with protein degradation and synthesis according to the functional categorization. Protein-degradation-related proteins mainly consisted of the proteasome degradation pathway. The cell death was significantly higher in the root tips of soybean under the combined treatment compared to flooding stress. Accumulation of calnexin/calreticulin and glycoproteins was significantly increased under flooding with silver NPs, nicotinic acid, and KNO. Growth of soybean seedlings with silver NPs, nicotinic acid, and KNO was improved under flooding stress. These results suggest that the combined mixture of silver NPs, nicotinic acid, and KNO causes positive effects on soybean seedling by regulating the protein quality control for the mis-folded proteins in the endoplasmic reticulum. Therefore, it might improve the growth of soybean under flooding stress.
大量使用银纳米粒子（NPs）在农产品中导致它们与环境中的其他化学物质相互作用。为了研究银纳米粒子与烟酸和硝酸钾（KNO）的联合效应，使用了无凝胶/无标记的蛋白质组学技术。银纳米粒子与烟酸和 KNO 混合后，大豆的根长/重和下胚轴长/重增加。在总共鉴定出的 6340 种蛋白质中，有 351 种蛋白质发生了显著变化，其中 247 种和 104 种蛋白质分别增加和减少。根据功能分类，差异变化的蛋白质主要与蛋白质降解和合成有关。与蛋白质降解相关的蛋白质主要由蛋白酶体降解途径组成。与单独的水淹胁迫相比，在联合处理下大豆根尖的细胞死亡明显更高。在银纳米粒子、烟酸和 KNO 存在下的水淹条件下，钙连蛋白/钙网蛋白和糖蛋白的积累显著增加。在水淹胁迫下，添加银纳米粒子、烟酸和 KNO 的大豆幼苗生长得到改善。这些结果表明，银纳米粒子、烟酸和 KNO 的联合混合物通过调节内质网中错误折叠蛋白质的蛋白质质量控制，对大豆幼苗产生积极影响。因此，它可能会改善大豆在水淹胁迫下的生长。
