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红薯作为开发用于培养乳酸菌的培养基的一种基本成分。

Sweet potatoes as a basic component in developing a medium for the cultivation of lactobacilli.

作者信息

Hayek Saeed A, Shahbazi Aboghasem, Awaisheh Saddam S, Shah Nagendra P, Ibrahim Salam A

机构信息

Energy and Environmental Systems, North Carolina Agricultural and Technical State University.

出版信息

Biosci Biotechnol Biochem. 2013;77(11):2248-54. doi: 10.1271/bbb.130508. Epub 2013 Nov 7.

Abstract

A sweet potato medium (SPM) was formed with extract from baked sweet potatoes supplemented with 0, 4, or 8 g/L of each nitrogen source (beef extract, yeast extract, and proteose peptone #3) to form SPM1, SPM2, and SPM3 respectively. Lactobacilli MRS was used as control medium. Ten Lactobacillus strains containing an average of 2.34 ± 0.29 log CFU/mL were inoculated individually into batches of MRS, SPM1, SPM2, and SPM3. The growth patterns for the tested Lactobacillus strains growing in SPM2 and SPM3 were found to be similar to that in MRS. The average final population after 24 h of incubation in MRS, SPM2, and SPM3 reached 10.41 ± 0.35, 10.59 ± 0.27, and 10.72 ± 0.19 log CFU/mL respectively. SPM2 and SPM3 maintained higher pH values throughout the incubation period than MRS. These findings indicate that SPM2 can be a suitable medium for the growth of Lactobacillus and can provide an alternative at low-cost.

摘要

用烤红薯提取物分别添加0、4或8 g/L的每种氮源(牛肉膏、酵母提取物和蛋白胨#3)制成红薯培养基(SPM),分别形成SPM1、SPM2和SPM3。乳酸杆菌MRS用作对照培养基。将平均含有2.34±0.29 log CFU/mL的10株乳酸杆菌菌株分别接种到一批MRS、SPM1、SPM2和SPM3中。发现测试的乳酸杆菌菌株在SPM2和SPM3中的生长模式与在MRS中的相似。在MRS、SPM2和SPM3中培养24小时后的平均最终菌数分别达到10.41±0.35、10.59±0.27和10.72±0.19 log CFU/mL。在整个培养期间,SPM2和SPM3的pH值高于MRS。这些发现表明,SPM2可以作为乳酸杆菌生长的合适培养基,并且可以低成本提供替代方案。

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