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弯曲杆菌在富马酸 - 丙酮酸培养基或添加了乳制品、肉类或大豆提取物及蛋白胨的培养基中需氧培养时的生长情况。

Growth of Campylobacter incubated aerobically in fumarate-pyruvate media or media supplemented with dairy, meat, or soy extracts and peptones.

作者信息

Hinton Arthur

机构信息

Poultry Microbiological Safety and Processing Unit, U. S. National Poultry Research Center, Agricultural Research Service, United States Department of Agriculture, 950 College Station Road, Russell Research Center, Athens, GA, 30605, USA.

出版信息

Food Microbiol. 2016 Sep;58:23-8. doi: 10.1016/j.fm.2016.03.010. Epub 2016 Mar 19.

Abstract

The ability of Campylobacter to grow aerobically in media supplemented with fumarate-pyruvate or with dairy, meat, or soy extracts or peptones was examined. Optical densities (OD) of Campylobacter cultured in basal media, media supplemented with fumarate-pyruvate or with 1.0, 2.5, 5.0, or 7.5% beef extract was measured. Growth was also compared in media supplemented with other extracts or peptones. Finally, cfu/mL of Campylobacter recovered from basal media or media supplemented with fumarate-pyruvate, casamino acids, beef extract, soytone, or beef extract and soytone was determined. Results indicated that OD of cultures grown in media supplemented with fumarate-pyruvate or with 5.0 or 7.5% beef extract were higher than OD of isolates grown in basal media or media supplemented with lower concentrations of beef extract. Highest OD were produced by isolates grown in media supplemented with beef extract, peptone from meat, polypeptone, proteose peptone, or soytone. Also, more cfu/mL were recovered from media with fumarate-pyruvate, beef extract, soytone, or beef extract-soytone than from basal media or media with casamino acids. Findings indicate that media supplemented with organic acids, vitamins, and minerals and media supplemented with extracts or peptones containing these metabolites can support aerobic growth of Campylobacter.

摘要

研究了弯曲杆菌在添加富马酸 - 丙酮酸、乳制品、肉类或大豆提取物或蛋白胨的培养基中需氧生长的能力。测量了在基础培养基、添加富马酸 - 丙酮酸或1.0%、2.5%、5.0%或7.5%牛肉提取物的培养基中培养的弯曲杆菌的光密度(OD)。还比较了在添加其他提取物或蛋白胨的培养基中的生长情况。最后,测定了从基础培养基或添加富马酸 - 丙酮酸、酪蛋白氨基酸、牛肉提取物、大豆蛋白胨或牛肉提取物和大豆蛋白胨的培养基中回收的弯曲杆菌的cfu/mL。结果表明,在添加富马酸 - 丙酮酸或5.0%或7.5%牛肉提取物的培养基中生长的培养物的OD高于在基础培养基或添加较低浓度牛肉提取物的培养基中生长的分离株的OD。在添加牛肉提取物、肉蛋白胨、聚蛋白胨、蛋白胨或大豆蛋白胨的培养基中生长的分离株产生的OD最高。此外,从含有富马酸 - 丙酮酸、牛肉提取物、大豆蛋白胨或牛肉提取物 - 大豆蛋白胨的培养基中回收的cfu/mL比从基础培养基或含有酪蛋白氨基酸的培养基中更多。研究结果表明,添加有机酸、维生素和矿物质的培养基以及添加含有这些代谢物的提取物或蛋白胨的培养基可以支持弯曲杆菌的需氧生长。

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