Takahashi Haruya, Suzuki Hideyuki, Suda Kunihiro, Yamazaki Yota, Takino Akihiro, Kim Young-Il, Goto Tsuyoshi, Iijima Yoko, Aoki Koh, Shibata Daisuke, Takahashi Nobuyuki, Kawada Teruo
Laboratory of Molecular Functions of Food, Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University.
Biosci Biotechnol Biochem. 2013;77(11):2288-93. doi: 10.1271/bbb.130572. Epub 2013 Nov 7.
A change in the free fatty acid (FFA) profile reflects an alteration in the lipid metabolism of peripheral tissue. A high-throughput quantitative analysis method for individual FFAs therefore needs to be established. We report here an optimized LC-MS assay for a high-throughput and high-sensitivity analysis of the 10 major long-chain FFAs in mouse plasma and liver. This assay enables quantification of individual FFAs by using trace amounts of samples (2 µL of plasma and 10 mg of liver tissue). We apply this method to analyze the FFA profile of plasma and liver samples from an obese mouse model treated with bezafibrate, the peroxisome proliferator-activated receptor α (PPARα) agonist, and show a change in the FFA profile, particularly in the palmitoleic and oleic acid contents. This assay is useful for quantifying individual FFAs and helpful for monitoring the condition of lipid metabolism.
游离脂肪酸（FFA）谱的变化反映了外周组织脂质代谢的改变。因此，需要建立一种针对单个FFA的高通量定量分析方法。我们在此报告一种优化的液相色谱-质谱（LC-MS）测定法，用于对小鼠血浆和肝脏中的10种主要长链FFA进行高通量和高灵敏度分析。该测定法能够通过使用微量样品（2μL血浆和10mg肝脏组织）对单个FFA进行定量。我们应用此方法分析了用苯扎贝特（过氧化物酶体增殖物激活受体α（PPARα）激动剂）治疗的肥胖小鼠模型的血浆和肝脏样品的FFA谱，并显示出FFA谱的变化，特别是棕榈油酸和油酸含量的变化。该测定法可用于定量单个FFA，并有助于监测脂质代谢状况。
