Laboratory of Genetics, Faculty of Agriculture, Kyoto University, Sakyo-ku, 606-01, Kyoto, Japan.
Theor Appl Genet. 1992 Aug;84(5-6):535-43. doi: 10.1007/BF00224149.
A 4.1-kb DNA clone (pTag546), which when used as a probe produces hypervariable DNA fingerprints in common wheat, was found among the genomic clones of Triticum aestivum cv 'Chinese Spring'. Nulli-tetrasomic analyses revealed that the sequences hybridizing to this clone were located at 12 loci on ten chromosomes of the A, B, and D genomes of common wheat. The complete nucleotide sequence of pTag546 was shown to have a transposable element-like structure within it, though no open reading frame was detected. The sequences located in the A and D genomes were assumed to have been derived from the B genome by transposition. Using this clone as a probe, we were able to identify 56 common wheat cultivars, some of which are closely related, by their DNA fingerprints. This suggests that pTag 546 will be useful for cultivar identification as well as for germ plasm evaluation in wheat.
一个 4.1kb 的 DNA 克隆（pTag546），当用作探针时，会在普通小麦中产生高度可变的 DNA 指纹图谱，在普通小麦 cv 'Chinese Spring' 的基因组克隆中被发现。单体-四体分析表明，与该克隆杂交的序列位于普通小麦 A、B 和 D 基因组的十个染色体的 12 个位点上。尽管没有检测到开放阅读框，但 pTag546 的完整核苷酸序列显示出具有转座元件样结构。位于 A 和 D 基因组中的序列被假定是通过转座从 B 基因组衍生而来的。使用这个克隆作为探针，我们能够通过 DNA 指纹图谱识别出 56 个普通小麦品种，其中一些品种是密切相关的。这表明 pTag546 将对小麦品种鉴定以及种质评估有用。
