Biology Department, McGill University, Stewart Biology Building 1205 Ave. Dr. Penfield, H3A 1B1, Montreal, Quebec, Canada.
Theor Appl Genet. 1992 Aug;84(5-6):643-55. doi: 10.1007/BF00224164.
Relationships among 18 peroxidases amino acid sequences of animal, microbial and plant origin were examined using optimum alignment of all pairwise sequence combinations to generate a total distance matrix. The matrix was used to cluster the sequences with complete linkage (farthest neighbour) procedures. Specific distances were calculated from the total distances matrix. The patterns of specific distances for each sequence were compared to evaluate the relationships between sequences, check their significance and construct subgroups of related sequences. The results were compared with those from clustering and its resultant dendrogram; good agreement was achieved. The 18 sequences fell into two principal groups, plant peroxidases and animal/microbial peroxidases. Within the plant peroxidases four subgroups were detected; the animal/microbial peroxidases formed a fifth subgroup. Profiles were constructed for the subgroups from lists of matching amino acids generated by the alignment calculations. Superimposed lists were realigned to recognise conserved areas and elements. Individual subgroup profiles for the plant peroxidases were then combined into a single profile which in turn was combined with profiles from the animal/microbial peroxidases. The final profile suggested that numerous sequence features (motifs) were common to peroxidases of widely different function and origins.
采用所有成对序列组合的最佳比对来生成总距离矩阵，检查了动物、微生物和植物来源的 18 种过氧化物酶氨基酸序列之间的关系。该矩阵用于使用完全连锁（最远邻居）程序对序列进行聚类。从总距离矩阵中计算出特定距离。比较每个序列的特定距离模式，以评估序列之间的关系，检查其显著性，并构建相关序列的子组。将结果与聚类及其产生的系统发育树进行比较；结果吻合较好。18 个序列分为两组，一组是植物过氧化物酶，另一组是动物/微生物过氧化物酶。在植物过氧化物酶中检测到四个亚组；动物/微生物过氧化物酶形成第五个亚组。从比对计算生成的匹配氨基酸列表为亚组构建轮廓。将叠加的列表重新对齐以识别保守区域和元素。然后将植物过氧化物酶的各个亚组轮廓组合成一个单一的轮廓，该轮廓再与动物/微生物过氧化物酶的轮廓组合。最终的轮廓表明，许多序列特征（基序）在功能和来源广泛不同的过氧化物酶中是共同的。
