Feurle G E, Büchler M, Helmstädter V
Acta Histochem. 1985;77(2):151-8. doi: 10.1016/S0065-1281(85)80076-3.
Extrapancreatic pancreatic polypeptide (PP) was quantified by determination of PP-cell density using antibodies to human PP (hPP) and bovine PP (bPP) in stomach, duodenum, jejunum, ileum, and colon of the dog. Further, these organs of 3 beagles were extracted and subjected to radioimmunoassay again using 2 different antisera (one directed against bPP, the other against hPP). Purification of these extracts were performed by Sephadex chromatography. We found bPP immunoreactive cells in stomach, small and large bowel, but hPP immunoreactive cells, besides in the pancreas, only in stomach and duodenum, thus demonstrating heterogeneity of extrapancreatic PP. Extraction and chromatography confirm the presence of PP immunoreactivity in the pancreas, stomach, small and large intestine. The observation of a postprandial rise of serum PP levels in a man with total duodenopancreatectomy suggests that the extrapancreatic PP cell may release PP.
通过使用抗人胰多肽（hPP）和抗牛胰多肽（bPP）抗体测定PP细胞密度，对犬的胃、十二指肠、空肠、回肠和结肠中的胰腺外胰多肽（PP）进行定量。此外，提取3只比格犬的这些器官，再使用2种不同的抗血清（一种针对bPP，另一种针对hPP）进行放射免疫测定。通过葡聚糖凝胶色谱法对这些提取物进行纯化。我们在胃、小肠和大肠中发现了bPP免疫反应性细胞，但hPP免疫反应性细胞，除了在胰腺中，仅在胃和十二指肠中发现，从而证明了胰腺外PP的异质性。提取和色谱分析证实了胰腺、胃、小肠和大肠中存在PP免疫反应性。在一名接受全十二指肠胰腺切除术的男性中观察到餐后血清PP水平升高，这表明胰腺外PP细胞可能释放PP。
