Feurle G E, Müller B, Rix E
Medizinische Poliklinik, University of Heidelberg, F R Germany.
Gut. 1987;28 Suppl(Suppl):19-23. doi: 10.1136/gut.28.suppl.19.
We investigated whether chronic subcutaneous infusion of neurotensin during 14 days would affect pancreatic and gastric growth of rats. In another experiment, neurotensin (836 pmol/kg) was injected intraperitoneally three times a day for three days in 12 rats. Thereafter, pancreatic DNA and in vitro incorporation of 3H-thymidine into pancreatic DNA was determined. Long term infusion of 282 pmol/kg neurotensin induced an increase of pancreatic weight, DNA, and pancreatic polypeptide, whereas pancreatic protein, RNA, amylase and lipase contents were not increased. In relation to DNA, even these parameters were significantly depressed. Insulin remained unchanged. Neurotensin, therefore, caused hyperplasia of the pancreas. Intraperitoneal injection of neurotensin induced an increase of pancreatic DNA content and stimulated 3H-thymidine incorporation into DNA, whereas caerulein only augmented 3H-thymidine incorporation. Moreover, long term neurotensin infusion led to a rise in protein concentration and an increase in the thickness of the gastric antrum; antral DNA concentration was insignificantly stimulated. Neurotensin, therefore, can act as a trophic factor on pancreas and gastric antrum of the rat.
我们研究了连续14天皮下慢性输注神经降压素是否会影响大鼠胰腺和胃的生长。在另一项实验中，对12只大鼠每天腹腔注射3次神经降压素（836 pmol/kg），持续3天。此后，测定胰腺DNA以及3H-胸腺嘧啶核苷在体外掺入胰腺DNA的情况。长期输注282 pmol/kg神经降压素可导致胰腺重量、DNA和胰腺多肽增加，而胰腺蛋白质、RNA、淀粉酶和脂肪酶含量并未增加。相对于DNA而言，甚至这些参数也显著降低。胰岛素水平保持不变。因此，神经降压素可引起胰腺增生。腹腔注射神经降压素可导致胰腺DNA含量增加，并刺激3H-胸腺嘧啶核苷掺入DNA，而蛙皮素仅增强3H-胸腺嘧啶核苷的掺入。此外，长期输注神经降压素会导致蛋白质浓度升高以及胃窦厚度增加；胃窦DNA浓度受到的刺激不显著。因此，神经降压素可作为大鼠胰腺和胃窦的营养因子。
